FLAGIPT1
FLAG® Immunoprecipitation Kit
Sinónimos:
Anti-ddddk, Anti-dykddddk
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About This Item
Productos recomendados
uso
kit sufficient for 50 reactions
Nivel de calidad
clases químicas de analitos
proteins (FLAG® Affinity Gels)
técnicas
immunoprecipitation (IP): suitable
Condiciones de envío
wet ice
temp. de almacenamiento
−20°C
Descripción general
Sigma′s FLAG Immunoprecipitation kit allows a rapid and efficient immunoprecipitation and elution of an active FLAG-tagged protein. The immunoprecipitation is performed with the ANTI-FLAG M2 affinity gel, which is a highly specific monoclonal antibody covalently attached to agarose resin. The use of affinity resin allows an efficient binding of FLAG-tagged proteins without the need for preliminary steps and calibrations. The immunoprecipitated FLAG-tagged proteins can be efficiently eluted from the resin with acidic conditions or by competition with FLAG peptide. The immunoprecipitated proteins can be detected for their size, post-translational modifications, and interactions on gel electrophoresis, and by activity assays.
Aplicación
Learn more product details in our FLAG® application portal.
Características y beneficios
- Utilizes highly specific ANTI-FLAG M2 affinity gel
- No preliminary steps or calibrations
- 3X FLAG™ peptide provides efficient and gentle elution through direct competition
Otras notas
All prodedures to be performed at 2-8°C.
Información legal
3xFLAG is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Producto relacionado
Referencia del producto
Descripción
Precios
Palabra de señalización
Warning
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Aquatic Chronic 3 - Eye Irrit. 2
Código de clase de almacenamiento
10 - Combustible liquids
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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