SK-N-AS

94092302, human nerve (bone marrow metastasis), Polygonal

• Código UNSPSC: 41106514

Propiedades

• product name: SK-N-AS, 94092302
• origen biológico: human nerve (bone marrow metastasis)
• modo de crecimiento: Adherent
• cariotipo: 46
• morfología: Polygonal
• productos: Not specified
• receptores: Vimentin
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: metastasis
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human neuroblastoma

Descripción línea celular

Derived from a female patient with neuroblastoma from the metastasis of the bone marrow. Tumourigenic in nude mice.

Aplicación

Neuroblastoma differentiation studies

Perfil del ADN

STR-PCR Data: Amelogenin: X
CSF1PO: 10,12
D13S317: 9
D16S539: 14
D5S818: 11,12
D7S820: 11,13
THO1: 9.3
TPOX: 11,12
vWA: 16,17

Medio de cultivo

DMEM or EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 2-4 x 10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Otras notas

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