I-10

89031605, mouse testis, Epithelial

• Código UNSPSC: 41106514

Propiedades

• product name: I-10, 89031605
• origen biológico: mouse testis
• modo de crecimiento: Adherent
• cariotipo: 2n = 40, hyperdiploid
• morfología: Epithelial
• productos: Steroids
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Mouse BALB/c Leydig cell testicular tumour

Descripción línea celular

Derived from a Leydig cell testicular tumour of a BALB/c mouse. The cells secrete steroids; this increases at saturation density. Secretion should be retained for over 50 passages. Exposure to 0.00001M cAMP stimulates secretion 4-5 fold. The major products are progesterone and its derivative 20 α-hydroxy-4-pregnen-3-one. It does not respond to interstitial cell stimulating hormone (ICSH) but is stimulated by cyclic adenosine monophosphate (AMP).

Aplicación

Tumourigenicity studies

Medio de cultivo

Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 6x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

Otras notas

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