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77618

Sigma-Aldrich

Fenol – cloroformo – mezcla de alcohol isoamílico

BioUltra, for molecular biology, 49.5:49.5:1

Sinónimos:

phenol:Chloroform:Isoamyl Alcohol

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About This Item

Número de CAS:
136112-00-0
Número MDL:
MFCD00133763
Código UNSPSC:
12352401
ID de la sustancia en PubChem:
329767817
NACRES:
NA.07

grado

for molecular biology

Nivel de calidad

100

Línea del producto

BioUltra

formulario

liquid

impurezas

DNases, none detected
 RNases, none detected
 phosphatases, none detected
 proteases, none detected

pH

7.7-8.3(H2O-phase, after extraction with H2O, 1:1)

densidad

1.29 g/mL at 20 °C

absorción

cut-off at 360 nm

temp. de almacenamiento

2-8°C

cadena SMILES

ClC(Cl)Cl.CC(C)CCO.Oc1ccccc1

InChI

1S/C6H6O.C5H12O.CHCl3/c7-6-4-2-1-3-5-6;1-5(2)3-4-6;2-1(3)4/h1-5,7H;5-6H,3-4H2,1-2H3;1H

Clave InChI

ZYWFEOZQIUMEGL-UHFFFAOYSA-N

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Categorías relacionadas

Aplicación


  • Simultaneous quantification of multiple RNA cargos co-loaded into nanoparticle-based delivery systems.: This study highlights the use of a phenol-chloroform-isoamyl alcohol mixture for the extraction and quantification of RNA cargos from nanoparticles, facilitating accurate delivery system analysis (Lokras et al., 2022).

  • Analysis of Circulating microRNAs and Their Post-Transcriptional Modifications in Cancer Serum by On-Line Solid-Phase Extraction-Capillary Electrophoresis-Mass Spectrometry.: This research utilized the phenol-chloroform-isoamyl alcohol mixture to extract microRNAs from serum samples, providing insights into cancer biomarkers (Pero-Gascon et al., 2018).

  • The application of alkaline lysis and pressure cycling technology in the differential extraction of DNA from sperm and epithelial cells recovered from cotton swabs.: The study demonstrates the effectiveness of using phenol-chloroform-isoamyl alcohol for DNA extraction in forensic applications, improving the analysis of mixed samples (Nori and McCord, 2015).

  • The critical role of DNA extraction for detection of mycobacteria in tissues.: This article emphasizes the necessity of phenol-chloroform-isoamyl alcohol extraction in accurately detecting mycobacterial DNA in tissue samples, enhancing diagnostic precision (Radomski et al., 2013).

Forma física

mixture of phenol; chloroform; isoamyl alcohol in the ratio of 49.5:49.5:1 (v/v/v); saturated with 100 mM TRIS pH 8.0; contains ~0.1% 8-hydroxyquinoline

Otras notas

Large- and small-scale phenol extractions

Palabra de señalización

Danger

Clasificaciones de peligro

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Repr. 2 - Skin Corr. 1B - STOT RE 1 Oral - STOT RE 2 - STOT SE 3

Órganos de actuación

Central nervous system, Liver,Kidney, Nervous system,Kidney,Liver,Skin

Código de clase de almacenamiento

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

177.8 °F - closed cup

Punto de inflamabilidad (°C)

81 °C - closed cup

Equipo de protección personal

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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