MABT1444

Anti-Tetranectin/CLEC3B Antibody, clone mAb10

• Sinónimos: Anti-CLEC3B Antibody, CLEC3B Detection Antibody, Clone mAb10 Anti-Tetranectin
• Código UNSPSC: 12352203
• NACRES: NA.74

Propiedades

• origen biológico: mouse
• Nivel de calidad: 200
• conjugado: unconjugated
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: mAb10, monoclonal
• mol peso: calculated mol wt 22.54 kDa; observed mol wt ~25 kDa
• purificado por: using protein G
• reactividad de especies: human
• envase: antibody small pack of 100 μg
• técnicas: ELISA: suitable; dot blot: suitable; western blot: suitable
• isotipo: IgG2bκ
• secuencia del epítopo: C-terminal half
• Nº de acceso Protein ID: NP_003269
• Nº de acceso UniProt: P05452
• Condiciones de envío: 2-8°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... CLEC3B(7123)

Descripción

Descripción general

Tetranectin (UniProt: P05452; also known as TN, C-type lectin domain family 3 member B, Plasminogen kringle 4-binding protein) is encoded by the CLEC3B (also known as TNA) gene (Gene ID: 7123) in human. Tetranectin is a tetrameric plasminogen-binding protein that is synthesized with a signal peptide (aa 1-21), which is subsequently removed in the mature form that is composed of three identical, noncovalently linked subunits each of 181 amino acids. The polypeptide consists of a short a-helical segment (aa 47-72) at the amino terminus that is involved in trimerization, followed by a C-type lectin domain (aa 77-198). It is present in both serum and the extracellular matrix and is expressed most abundantly in the lung. The blood concentrations of tetranectin in healthy adults are about 10-12 mg/mL. However, in septic subjects its levels are significantly reduced and supplementation with exogenous tetranectin confers dose-dependent protection against lethal endotoxemia and sepsis. Its levels are shown to be significantly reduced in patients with cardiac failure. Hence, it is also used as a biomarker for risk of heart failure. (Ref.: Chen, W., et al. (2020). Sci. Transl. Med. 12(539); eaaz3833; McDonald, K., et al. (2020). Sci. Rep. 10; 7507).

Especificidad

Clone mAb10 is a mouse monoclonal antibody that detects Tetranectin. It targets an epitope within 24 amino acids from the C-terminal half.

Inmunógeno

Purified human Tetranectin.

Aplicación

Quality Conrol Testing

Evaluated by Western Blotting in Human lung tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Tetranectin/CLEC3B in Human lung tissue lysate.

Tested Applications

Western Blotting Analysis: A representative lot detected Tetranectin/CLEC3B in Western Blotting applications (Chen, W., et al. (2020). Sci Transl Med. 12(539):eaaz3833).

Dot Blot: A representative lot detected Tetranectin/CLEC3B in Dot Blot applications (Chen, W., et al. (2020). Sci Transl Med. 12(539):eaaz3833).

ELISA Analysis: A representative lot detected Tetranectin/CLEC3B in ELISA applications (Chen, W., et al. (2020). Sci Transl Med. 12(539):eaaz3833).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Tetranectin/CLEC3B, clone mAb10, Cat. No. MABT1444, is a mouse monoclonal antibody that detects Tetranectin and is tested for use in Dot Blot, ELISA, and Western Blotting.

Forma física

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable