MABS2317

Anti-SIRPA/CD172a Antibody, clone SE12C3

• Sinónimos: Brain Ig-like molecule with tyrosine-based activation motifs, CD172 antigen-like family member A, CD172a, Inhibitory receptor SHPS-1, Macrophage fusion receptor MyD-1 antigen, SHP substrate 1, SHPS-1, Signal-regulatory protein alpha-1, Signal-regulatory protein alpha-2, Signal-regulatory protein alpha-3, Sirp-alpha-1, Sirp-alpha-2, Sirp-alpha-3, Tyrosine-protein phosphatase non-receptor type substrate 1, p84
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 200
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: SE12C3, monoclonal
• mol peso: calculated mol wt 55 kDa; observed mol wt ~N/A kDa
• purificado por: using protein G
• reactividad de especies: human
• envase: antibody small pack of 100
• técnicas: ELISA: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunoprecipitation (IP): suitable; inhibition assay: suitable
• isotipo: IgG1
• secuencia del epítopo: Extracellular domain
• Nº de acceso Protein ID: NP_001035111
• Nº de acceso UniProt: P78324
• temp. de almacenamiento: -10 to -25°C
• Información sobre el gen: human ... SIRPA(140885)

Descripción

Especificidad

Clone SE12C3 is a mouse monoclonal antibody that detects SIRPA/CD172a. It targets an epitope within the extracellular domain.

Inmunógeno

GST-tagged recombinant extracellular domain of human SIRPA/CD172a.

Aplicación

Quality Control Testing

Evaluated by Flow Cytometry in Human peripheral blood mononuclear cells (PBMC).

Flow Cytometry Analysis: 1.0 µg of this antibody detected SIRPA/CD172a in one million Human peripheral blood mononuclear cells (PBMC).

Tested Applications

Immunoprecipitation Analysis: A representative lot immunoprecipitated SIRPA/CD172a in Immunoprecipitation applications (Seiffert, M., et al. (1999). Blood. 94(11):3633-43).

Inhibition Assay: A representative lot inhibited the binding of soluble SIRPA to cells. (Seiffert, M., et al. (1999). Blood. 94(11):3633-43; Murata, Y., et al. (2018). Cancer Sci. 109(5):1300-1308).

Immunocytochemistry Analysis: A representative lot detected SIRPA/CD172a in Immunocytochemistry applications (Seiffert, M., et al. (1999). Blood. 94(11):3633-43; Murata, Y., et al. (2018). Cancer Sci. 109(5):1300-1308).

ELISA Analysis: A representative lot detected SIRPA/CD172a in ELISA applications (Murata, Y., et al. (2018). Cancer Sci. 109(5):1300-1308).

Flow Cytometry Analysis: A representative lot detected SIRPA/CD172a in Flow Cytometry applications (Seiffert, M., et al. (1999). Blood. 94(11):3633-43).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Descripción de destino

Tyrosine-protein phosphatase non-receptor type substrate 1 (UniProt: P78324; also known as SHP substrate 1, SHPS-1, Brain Ig-like molecule with tyrosine-based activation motifs, Bit, CD172 antigen-like family member A, Inhibitory receptor SHPS-1, Macrophage fusion receptor, MyD-1 antigen, Signal-regulatory protein alpha-1, Sirp-alpha-1, Signal-regulatory protein alpha-2, Sirp-alpha-2, Signal-regulatory protein alpha-3, Sirp-alpha-3, p84, CD172a) is encoded by the SIRPA (also known as BIT, MFR, MYD1, PTPNS1, SHPS1, SIRP) gene (Gene ID: 140885) in human. SIRPA/CD172a is a single-pass type I membrane protein of the Ig superfamily that is ubiquitously expressed with high expression reported in brain. It is also detected on myeloid cells, but not on T cells. SIRPA is synthesized with a signal peptide (aa 1-30), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 31-373), a transmembrane domain (aa 374-394) and a cytoplasmic domain (aa 395-504). The extracellular region contains three Ig-like loops. The most distal loop (D1) contains and Ig-like V type domain (aa 32-137) whereas the two membrane proximal loops contain Ig-like C1 type domains (aa 148-247 and 254-348). SIRPA serves as an immunoglobulin-like cell surface receptor for CD47, and their interactions is reported to mediate negative regulation of several monocyte/ macrophage functions. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. SIRP alpha also acts as a docking protein and induces translocation of PTPN6, PTPN11, and other binding partners from the cytosol to the plasma membrane. SIRPA is phosphorylated on tyrosine residues in response to stimulation with EGF, growth hormone, insulin, and platelet derived growth factor. (Ref.: Murata, Y., et al., ( 2018). Cancer Sci. 109(5); 1300-1308; Lee, WY et al., (2007). J. Immunol.; 179(11); 7741-7750; Seiffert, M., et al (1999). Blood. 94(11); 3633-3643).

Forma física

Purified mouse monoclonal antibody IgG1 in PBS without preservatives.

Reconstitución

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Almacenamiento y estabilidad

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable