MABF3107

Anti-ULBP2 Antibody, clone BUMO1

• Sinónimos: ALCAN-alpha, N2DL-2, NKG2D ligand 2, NKG2DL2, Retinoic acid early transcript 1H, UL16-binding protein 2
• Código UNSPSC: 12352203
• NACRES: NA.43

Propiedades

• origen biológico: mouse
• Nivel de calidad: 200
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: BUMO1, monoclonal
• mol peso: calculated mol wt 27.37 kDa; observed mol wt ~N/A kDa
• purificado por: using protein G
• reactividad de especies: human
• envase: antibody small pack of 100
• técnicas: ELISA: suitable; flow cytometry: suitable
• isotipo: IgG1κ
• secuencia del epítopo: Unknown
• Nº de acceso Protein ID: NP_079493
• Nº de acceso UniProt: Q9BZM5
• temp. de almacenamiento: 2-8°C
• Información sobre el gen: human ... ULBP2(80328)

Descripción

Especificidad

Clone BUMO1 is a mouse monoclonal antibody that detects UL16-binding protein 2 (ULBP2).

Inmunógeno

p815 murine mastocytoma cells expressing human ULBP2.

Aplicación

Quality Control Testing

Evaluated by Flow Cytometry in C1R cells expressing ULBP2.

Flow Cytometry Analysis: 1.0 µg of this antibody detected ULBP2 in one million C1R cells expressing ULBP2.

Tested Applications

ELISA Analysis: A representative lot detected ULBP2 in ELISA applications (Waldhauer, I., et al. (2006). Cancer Res. 66(5):2520-6; Weil, S., et al. (2017). Front Immunol. 10:8:387).

Flow Cytometry Analysis: A representative lot detected ULBP2 in Flow Cytometry applications (Welte, S.A., et al. (2003). Eur J Immunol. 33(1):194-203; Waldhauer, I., et al. (2006). Cancer Res. 66(5):2520-6; Heinemann, A., et al. (2012). Cancer Res. 72(2):460-71).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Descripción de destino

UL16-binding protein 2 (UniProt: Q9BZM5; also known as ALCAN-alpha, NKG2D ligand 2, N2DL-2, NKG2DL2, Retinoic acid early transcript 1H) is encoded by the ULBP2 (also known as N2DL2, RAET1H, UNQ463/PRO791) gene (Gene ID: 80328) in human. The UL16-binding proteins (ULBPs) belong to the family of MHC class I-related molecules that are targets of human CMV glycoprotein. ULBP2 is a GPI-anchor glycoprotein that is synthesized with a signal peptide (aa 1-25) and a propeptide (aa 217-246), which are subsequently cleaved off to produce the mature form. ULBP2 serves as a ligand of the activating immunoreceptor NKG2D and mediates natural kill (NK) cell cytotoxicity. It is detected in cell membrane and endoplasmic reticulum. A secreted for has also been described. Its expression is observed in cancer cells and in the fetus, but not in normal tissues. Tumor cells are reported to release the soluble form of ULBP2 by the action of metalloproteases, however, unlike membrane bound form, it does not down-regulate NKG2D on NK cells. Soluble form of ULBP2 is frequently detected in sera of patients with hematopoietic malignancies. NKG2D, frequently expressed on tumor cells, stimulate tumor immunity. Soluble forms of ULBPs are also reported to induce protein tyrosine phosphorylation, activation of Janus kinase 2, MAP kinase, and PI-3 kinase/Akt signaling. Clone BUMO-1 specifically detects ULBP2 and is suitable for multiple immunodetection applications. (Ref.: Waldhauer, I., and Steinle, A. (2006). Cancer Res 66(5); 2520-2526; Welte, SA., et al. (2003). Eur. J. Immunol. 33(1); 194-203; Sutherland, CL., et al. (2002). J. Immunol. 168(2); 671-679).

Forma física

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Reconstitución

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Almacenamiento y estabilidad

Recommended storage: +2°C to +8°C.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable