MABF2045

Anti-Siglec-H Antibody, clone 551

clone 551, from rat

• Sinónimos: Sialic acid binding Ig-like lectin H, Siglech
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rat
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: 551, monoclonal
• reactividad de especies: mouse
• envase: antibody small pack of 25 μg
• técnicas: flow cytometry: suitable; immunohistochemistry: suitable
• isotipo: IgG1κ
• Nº de acceso NCBI: NP_848821.2
• Nº de acceso UniProt: Q3Y597
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: mouse ... Siglech(233274)

Descripción

Descripción general

Siglec-H (UniProt: Q3Y597; also known as Sialic acid binding Ig-like lectin H) is encoded by the Siglech gene (Gene ID: 233274) in murine species. Siglecs are sialic acid-binding immunoglobulin-like lectins that are expressed mostly in the immune system and may be involved in both adhesive and signaling functions. Siglec-H is a type I, CD33-related siglec-like transmembrane glycoprotein that is synthesized with a signal peptide (aa 1-18), which is subsequently cleaved off in the mature form. The mature form has to ig-like domains (aa 32-141 and 151-234) in its extracellular region. Unlike other CD33-related siglecs, it lacks tyrosine-based signaling motifs in its cytoplasmic tail. Siglec-H is shown to be expressed on plasmaocytoid dendritic cells (pDCs) and can serve as a reliable marker for these cells. Siglec-H is also reported to serve as an endocytic receptor expressed on murine pDCs. Action of Siglec-H depends on a 12 kDa DNAX-activating protein (DAP). (Ref.: Zhang, J., et al. (2006). Blood 107(9); 3600-3608; Yun, TJ., et al., (2016), Cell Metabolism 23(5); 852-866).

Especificidad

Clone 551 is a rat monoclonal antibody that detects murine Siglec-H. It targets an epitope with in the extracellular domain.

Inmunógeno

Bone marrow-derived Type 1 interferon-producing cells.

Epitope: extracellular domain

Aplicación

Anti-Siglec-H, clone 551, Cat. No. MABF2045, is a rat monoclonal antibody that detects Sialic acid binding Ig-like lectin H and has been tested for use in Flow Cytometry and Immunohistochemistry.

Immunohistochemistry Analysis: A representative lot detected Siglec-H in Immunohistochemistry applications (Yun, T.J., et. al. (2016). Cell Metab. 23(5):852-66).

Flow Cytometry Analysis: A representative lot detected Siglec-H in Flow Cytometry applications (Yun, T.J., et. al. (2016). Cell Metab. 23(5):852-66).

Research Category
Inflammation & Immunology

Calidad

Evaluated by Immunohistochemistry in frozen mouse spleen tissue sections.

Immunohistochemistry Analysis: A 1:25 dilution of this antibody detected Siglec-H in frozen mouse spleen tissue sections.

Descripción de destino

34.35 kDa calculated.

Forma física

Format: Purified

Protein G purified

Purified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable