ABN1660-I

Anti-S-Opsin

• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 200
• conjugado: unconjugated
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: Unknown, polyclonal
• mol peso: calculated mol wt 38.92 kDa; observed mol wt ~37 kDa
• purificado por: affinity chromatography
• reactividad de especies: mouse
• reactividad de especies (predicha por homología): rat
• envase: antibody small pack of 100 μg
• técnicas: immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• isotipo: IgG
• secuencia del epítopo: C-terminal half
• Nº de acceso Protein ID: NP_031564
• Nº de acceso UniProt: P51491
• Condiciones de envío: ambient
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: rabbit ... Pun1sw(12057)

Descripción

Descripción general

Short-wave-sensitive opsin 1 (UniProt: P51491; also known as S opsin, Blue cone photoreceptor pigment, Blue-sensitive opsin, BOP, Short wavelength-sensitive cone opsin) is encoded by the Opn1sw (also known as Bcp) gene (Gene ID: 12057) in murine species. Opsins are G-protein coupled protein with photoreceptor activity. Opsins contain seven transmembrane alpha-helical domains connected by three extra-cellular and three cytoplasmic loops. They are light-sensitive proteins found in photoreceptor cells of the retina. Based on sequence homology, the opsins are classified into six subfamilies: the vertebrate opsin/encephalopsin subfamily, the Go opsin subfamily, the Gs opsin subfamily, the invertebrate Gq opsin subfamily, the photoisomerase subfamily, and the neuropsin subfamily. They share less than 20 percent identity between subfamilies. Opsins provide the necessary environment for the absorption of light at a particular wavelength. Therefore, by providing a different opsin to the retinal, organisms can sense light of different wavelengths. S-opsin is a multi-pass membrane protein that is expressed in cone photoreceptor cells. They are light-absorbing molecules that mediate vision and contain an apoprotein, opsin, which is covalently linked to cis-retinal. It can increase spectral sensitivity in dim light.

Especificidad

This rabbit polyclonal antibody specifically detects S-Opsin (short-wave-sensitive opsin 1). It targets an epitope within 17 amino acids from the C-terminal half.

Inmunógeno

KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal half of murine short-wave-sensitive opsin 1.

Aplicación

Quality Control Testing

Evaluated by Western Blotting in NRL knockout mouse retina lysate

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected S-Opsin in NRL knockout mouse retina tissue lysate.

Tested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected S-Opsin in mouse retina tissue sections.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected S-Opsin in Mouse Eye tissue lysate.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-S-Opsin , Cat. No. ABN1660-I, is a rabbit polyclonal antibody that detects S-Opsin (Short-wave-sensitive opsin 1) and is tested for use in Immunohistochemistry (Paraffin), Western Blotting, and Western Blotting using KO lysates.

Forma física

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide

Almacenamiento y estabilidad

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable