ABE477

Anti-BRD2 Antibody

from rabbit, purified by affinity chromatography

• Sinónimos: Bromodomain-containing protein 2, O27.1.1, Really interesting new gene 3 protein
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: human, rat
• técnicas: immunohistochemistry: suitable; western blot: suitable
• Nº de acceso NCBI: NP_001106653.1
• Nº de acceso UniProt: P25440
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... BRD2(6046)

Descripción

Descripción general

Bromodomain-containing protein 2 (BRD2) is a member of the BET subfamily of bromodomain proteins. These proteins contain two tandem N-terminal bromodomains and a single C-terminal extra-terminal domain. Studies have shown BRD2 to be associated with several regulators of transcription (eg. TFIID and Swi/Snf complexes) in addition to its involvement in guiding cell cycle gene expression through its binding to multiple E2F. BRD2 is hypothesized to function in mammalian development by regulating chromatin structure and transcription. Consistent with this hypothesis, studies have shown BRD2 to bind histone H4 via acetylated Lys12. BRD2 mutants have been associated with increased susceptibility to juvenile myoclonic epilepsy.

Especificidad

This antibody is expected to cross react with isoforms 1 and 2 of BRD2, based upon sequence homology.

Inmunógeno

GST-tagged recombinant protein corresponding to a region near the C-terminus of human BRD2.

Aplicación

Anti-BRD2 Antibody is a highly specific rabbit polyclonal antibody, that targets BRD2 & has been tested in western blotting & IHC.

Immunohistochemistry Analysis: A 1:500 dilution of this antibody from a representative lot detected BRD2 in human testis tissues.

Immunohistochemistry Analysis: A 1:2,000 and a 1:500 dilution from a representative lot detected BRD2 in rat testis tissues.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Calidad

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 0.25 µg/mL of this antibody detected BRD2 in 10 µg of Jurkat cell lysate.

Descripción de destino

~110 kDa observed. Uniprot describes two isoforms at 88 kDa (isoform 1) and 92 kDa (isoform 2). Uncharacterized band(s) may be observed in some cell lysates.

Forma física

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable