ABC974
Anti-ATG8 Antibody
from rabbit, purified by affinity chromatography
Sinónimos:
Atg8, Atg8a, Atg8b, Autophagy-specific gene 8a, Autophagy-specific gene 8b
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
Productos recomendados
origen biológico
rabbit
forma del anticuerpo
affinity isolated antibody
tipo de anticuerpo
primary antibodies
clon
polyclonal
purificado por
affinity chromatography
reactividad de especies
Drosophila
reactividad de especies (predicha por homología)
Caenorhabditis elegans (based on 100% sequence homology)
técnicas
western blot: suitable
Condiciones de envío
wet ice
modificación del objetivo postraduccional
unmodified
Información sobre el gen
fruit fly ... Atg8A(32001)
Descripción general
Autophagy-specific gene 8a and 8b (UniProt Q9W2S2 and Q9VEG5, respectively) are encoded by the drosophila Atg8a (ORF Dmel_CG32672; Gene ID 32001) and Atg8b (ORF Dmel_CG12334; Gene ID 42132) gene, respectively. Autophagy is a fundamental an adaptive response to cellular stress caused by, for example, starvation and viral infection. Autophagy is a process conserved from yeast to human and involves the formation of double-membrane vesicles for the purpose of recycling nutrients by means of degrading the engulfed content. In resting cells, the majority of Atg8 is in an unlipidated form called Atg8-I. During autophagy, Atg8-I is conjugated with a lipid moiety, and the lipidated form (Atg8-II) is recruited to autophagosomal membranes. An elevated Atg8-II level therefore serves as an indicator of autophagy activation. LC3 is the mammalian ortholog of drosophila Atg8.
Especificidad
Reacts with the unlipidated (Atg8-I) and lipidated (Atg8-II) forms of drosophila Atg8a and Atg8b (Shelly, S., et al. (2009). Immunity. 30(4):588-598).
Inmunógeno
Epitope: Near N-terminus.
Linear peptide based on drosophila Atg8a and Atg8b sequences near the N-terminus.
Aplicación
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
This Anti-ATG8 Antibody is validated for use in Western Blotting for the detection of ATG8.
Western Blotting Analysis: A representative lot detected the induction of the lipidated Atg8-II band in wild-type flies upon Rift Valley fever virus (RVFV) infection, while an enhanced Atg8-II induction was observed in Toll-1-overexpressing flies and no Atg8-II induction by RVFV infection was seen among Toll-7 mutant flies (Moy, R.H., et al. (2014). Immunity. 40(1):51-65).
Western Blotting Analysis: A representative lot detected sdRNA-targeted downregulation of Atg8a and Atg8b in Drosophila S2 cells (Shelly, S., et al. (2009). Immunity. 30(4):588-598).
Western Blotting Analysis: A representative lot detected the 16 kDa Atg8-I band (unlipidated) as well as the serum starvation- and vesicular stomatitis virus (VSV) infection-induced 14 kDa Atg8-II band (lipidated) in Drosophila S2 cells (Shelly, S., et al. (2009). Immunity. 30(4):588-598).
Western Blotting Analysis: A representative lot detected sdRNA-targeted downregulation of Atg8a and Atg8b in Drosophila S2 cells (Shelly, S., et al. (2009). Immunity. 30(4):588-598).
Western Blotting Analysis: A representative lot detected the 16 kDa Atg8-I band (unlipidated) as well as the serum starvation- and vesicular stomatitis virus (VSV) infection-induced 14 kDa Atg8-II band (lipidated) in Drosophila S2 cells (Shelly, S., et al. (2009). Immunity. 30(4):588-598).
Calidad
Evaluated by Western Blotting in DL1 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected ATG8 in 10 µg of DL1 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected ATG8 in 10 µg of DL1 cell lysate.
Descripción de destino
~16/14 kDa observed. The upper band of the doublet represents the unlipidated Atg8-I form, while the lower band of the doublet represents the lipidated Atg8-II form that is upregulated upon autophagy induction.
Forma física
Affinity purified
Purified rabbit polyclonal antibody in PBS with 0.02% sodium azide.
Almacenamiento y estabilidad
Stable for 1 year at 2-8°C from date of receipt.
Otras notas
Concentration: Please refer to lot specific datasheet.
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Opcional
Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 2
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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