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AB5054

Sigma-Aldrich

Anti-Calretinin Antibody

serum, Chemicon®

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702

origen biológico

rabbit

Nivel de calidad

forma del anticuerpo

serum

clon

polyclonal

reactividad de especies

human, rat

fabricante / nombre comercial

Chemicon®

técnicas

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

Nº de acceso NCBI

Nº de acceso UniProt

Condiciones de envío

dry ice

Información sobre el gen

human ... CALB2(794)

Descripción general

Calretinin is a 29 kDa calcium binding protein that is expressed in central and peripheral nervous system and in many normal and pathological tissues. Calretinin is also considered an important diagnostic aid in the differential diagnosis of cystic and solid ameloblastic tumors. The rat and human calretinin exhibit 98% sequence homology and 91% homology to many other species. Calretinin is generally expressed with two other calcium binding proteins Calbindin D-29 and parvalbumin in CNS and other peripheral tissues. Calretinin antibodies have been used to detect a number of carcinomas by clinicopathological, immunohistochemical techniques including, epithelial malignant mesotheliomas, Ovarian yolk sac tumor, Adenomatoid tumors of the uterus etc.

Especificidad

Reactivity with other species has not been determined
Specific for calretinin. Recognizes both the calcium-bound and calcium-unbound conformations of calretinin by western blots.

Inmunógeno

Recombinant rat calretinin.

Aplicación

Research Category
Neuroscience
Research Sub Category
Signaling Neuroscience
This Anti-Calretinin Antibody is validated for use in IH, IH(P), WB for the detection of Calretinin.
Western blot:
1:1,000-1:2,000 of a previous lot was used.

Immunohistochemistry:
1:1,000-1:10,000
Antibody works in paraffin embedded tissues.

Optimal working dilutions must be determined by the end user.

Calidad

Immunohistochemistry(paraffin) Analysis:
Calretinin (cat. # AB5054) staining on Human Cerebellum. Tissue pretreated with Citrate, pH 6.0 plus. Antibody was diluted to 1:200 (5.0 µg/mL), using IHC-Select Detection with HRP-DAB. Immunoreactivity is seen as a nuclear and cytoplasmic punctate staining on neurons (brown).
Optimal Staining With Citrate Buffer Epitope Retrieval: Human Cerebellum.

Ligadura / enlace

Replaces: AB149

Forma física

Rabbit polyclonal serum contains no preservative.
Unpurified

Almacenamiento y estabilidad

Stable for 1 year at -20°C in undiluted aliquots from date of receipt.

Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Nota de análisis

Control
Rat brain cortex, Mesothelioma tissue, Human cerebellum.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Calcium-binding Protein Calretinin Immunoreactivity in the Dog Superior Colliculus.
Lee, Jea-Young, et al.
Acta Histochemica Et Cytochemica, 39, 125-138 (2006)
TRPV1 receptors modulate retinal development.
Mauro Leonelli,Daniel O Martins,Luiz R G Britto
International Journal of Developmental Neuroscience null
Repeated phencyclidine in monkeys results in loss of parvalbumin-containing axo-axonic projections in the prefrontal cortex.
Bret A Morrow, John D Elsworth, Robert H Roth
Psychopharmacology (Psychopharmacologia) null
Immunohistochemical and histochemical characteristics of the olfactory system of the guppy, Poecilia reticulata (Teleostei, Poecilidae).
Bettini S, Lazzari M, Ciani F, Franceschini V
Anatomical Record (Hoboken, N.J. : 2007) null
Allison Walter et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 24(9), 3360-3369 (2010-05-06)
This study evaluated the in vivo antitumor activity of grape-derived polyphenols. BALB/c mice were subcutaneously implanted with C26 colon carcinoma cells, and 2 d later they received either solvent or red wine polyphenols (RWPs) (100 mg/kg/d, human equivalent dose approximately

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