324405
Anti-Dopamine D₄ Receptor Rabbit pAb
lyophilized, Calbiochem®
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About This Item
Código UNSPSC:
12352203
NACRES:
NA.43
origen biológico
rabbit
Nivel de calidad
forma del anticuerpo
serum
tipo de anticuerpo
primary antibodies
clon
polyclonal
formulario
lyophilized
no contiene
preservative
reactividad de especies
human, rat
fabricante / nombre comercial
Calbiochem®
condiciones de almacenamiento
OK to freeze
isotipo
IgG
Condiciones de envío
ambient
temp. de almacenamiento
2-8°C
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... DRD4(1815)
rat ... Drd4(25432)
Descripción general
Anti-Dopamine D4 Receptor, rabbit polyclonal, recognizes ~48-51 kDa dopamine D4 receptor in rat brain. Does not cross-react with other dopamine receptors. It is validated for use in ELISA, WB & IHC.
Rabbit polyclonal antibody supplied as lyophilized, undiluted serum. Recognizes the ~48-51 kDa D4 dopamine receptor.
Recognizes the ~48-51 kDa dopamine D4 receptor in rat brain. Does not cross-react with other dopamine receptors.
Inmunógeno
Human
a synthetic peptide corresponding to amino acids 176-185 of human dopamine D₄ receptor
Aplicación
ELISA (see comments)
Immunoblotting (1:500)
Immunohistochemistry (1:10,000)
Immunoblotting (1:500)
Immunohistochemistry (1:10,000)
Advertencia
Toxicity: Standard Handling (A)
Forma física
Undiluted serum.
Reconstitución
Reconstitute the lyophilized antibody with 100 µl PBS containing 10 mg/ml BSA. For further dilution of the antiserum use a similar buffer formulation. Following reconstitution, refrigerate (4°C). Variables associated with assay conditions will dictate the proper working dilution.
Nota de análisis
Positive Control
Rat brain
Rat brain
Otras notas
Mignini, F., et al. 2000. J. Autonom. Pharmacol.20, 325.
This antibody has also been reported to work for ELISA. For blocking immunoblot membranes, use ONLY 4% goat serum or evaporated goat′s milk. For immunohistochemistry, a 1:10,000 dilution of the reconstituted serum demonstrated specific staining of rat brain sections including olfactory bulb glomeruli, cortex layer 4, cortex layers 2 and 3 (dendrite staining), cortex layer 5 (apical dendrite staining), and the dentate gyrus of the hippocampus (diffuse staining in the molecular layer, punctuate staining in the granule cell layer).
Información legal
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 2
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Alberto Ricci et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 50(8), 1091-1096 (2002-07-23)
Dopamine D4 receptors mediate inhibition of vasopressin-dependent sodium reabsorption by dopamine in collecting tubules. At present, the distribution of D4 receptors in other renal districts remains an open issue. The renal distribution of D4 receptor was assessed in normally innervated
Satoko Oda et al.
Frontiers in neuroanatomy, 17, 1111008-1111008 (2023-03-04)
Pial astrocytes, a cellular component of the cerebral cortex surface structure, are observed in a wide range of mammalian species. Despite being recognized as such, the functional potential of pial astrocytes has long been overlooked. Our previous research demonstrated that
Dana C Borcherding et al.
PloS one, 6(9), e25537-e25537 (2011-10-04)
Dopamine (DA) binds to five receptors (DAR), classified by their ability to increase (D1R-like) or decrease (D2R-like) cAMP. In humans, most DA circulates as dopamine sulfate (DA-S), which can be de-conjugated to bioactive DA by arylsulfatase A (ARSA). The objective
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