16-218

Anti-Phospho-Histone H3 (Ser10) Antibody, clone 3H10, Cy5 conjugate

clone 3H11, Upstate^®, from mouse

• Sinónimos: H3 histone, family 3A, H3S10P, Histone H3 (phospho S10), H3 histone, family 3A, H3 histone, family 3B, H3 histone, family 3B (H3.3B)
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• conjugado: CY5 conjugate
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: 3H11, monoclonal
• reactividad de especies: human
• fabricante / nombre comercial: Upstate^®
• técnicas: immunofluorescence: suitable; western blot: suitable
• isotipo: IgG1κ
• Nº de acceso NCBI: NM_003493.2
• Nº de acceso UniProt: Q16695
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: phosphorylation (pSer10)
• Información sobre el gen: human ... HIST1H3F(8968)

Descripción

Descripción general

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure.

The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Especificidad

Expected to cross react with all vertebrate species.

Histone H3 phosphorylated at Ser10

Inmunógeno

A proprietary immunogen based on a peptide sequence containing phospho-serine corresponding to residue 10 of human histone H3, clone 3H10. This is a Cy5 conjugate of Anti-phospho-Histone H3 (Ser10), clone 3H10, Catalog # 05-806.

Aplicación

Anti-Phospho-Histone H3 (Ser10) Antibody, clone 3H10, Cy5 Conjugate is a Mouse Monoclonal Antibody for detection of Histone H3 phosphorylated at serine 10. This purified mAb, also known as H3S10p,
is labeled with Cy5.5 NHS ester (Cy5) and validated in IF, WB.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Calidad

Routinely evaluated by immunoblot in acid extrated protein from mitotic HeLa cells treated with colcemid (17-306).

Descripción de destino

17kDa

Forma física

Protein G Purified

Almacenamiento y estabilidad

1 year at 4°C from date of shipment

Nota de análisis

Control
Acid extracts from colcemid treated HeLa cells (lane 2)

Información legal

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable