07-425-I

Anti-Neurogranin

from rabbit, purified by affinity chromatography

• Sinónimos: Ng, RC3
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: rat, human
• reactividad de especies (predicha por homología): goat (based on 100% sequence homology), bovine (based on 100% sequence homology), mouse (based on 100% sequence homology)
• envase: antibody small pack of 25 μg
• técnicas: immunohistochemistry: suitable (paraffin); western blot: suitable
• isotipo: IgG
• Nº de acceso NCBI: NP_001119653
• Nº de acceso UniProt: Q92686
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... NRGN(4900)

Descripción

Descripción general

Neurogranin (UniProt: Q92686; also known as Ng, RC3) is encoded by the NRGN gene (Gene ID: 4900) in human. Neurogranin is abundantly expressed in cerebral cortex, hippocampus, amygdala, and striatum. In neurons, it is found concentrated at dendritic spines. It acts as a third messenger and serves as a substrate for protein kinase C-mediated molecular cascades during synaptic development and remodeling. It contains an IQ domain (aa 26-347) and a collagen-like domain (aa 48-78). The IQ domain mediates its interaction with calmodulin and phosphatidic acid (PA). Neurogranin is intrinsically unstructured, however, upon binding with calmodulin, the IQ domain adopts a helical conformation. Neurogranin is phosphorylated at serine 36 by protein kinase C and following phosphorylation neurogranin is unable to bind either calmodulin or PA, which can lead to decline in memory-associated functions. Higher levels of neurogranin have also been reported in brains of elderly and is considered a factor in memory decline, independent of Alzheimer s disease. (Ref.: Javier Dı´ez-Guerra, F. (2010). Life. 62(8): 597-606; Casaletto, KB et al. (2017). Neurology 89(17); 1782-1788).

Especificidad

This rabbit polyclonal antibody sepecifically detects Neurogranin. It targets an epitope with in 12 amino acids from the C-terminal region.

Inmunógeno

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to 12 amino acids from the C-terminal region of human Neurogranin.

Aplicación

Anti-Neurogranin Antibody, Cat. No. 07-425-I, is a highly specific rabbit polyclonal antibody that targets Neurogranin and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Neurogranin in human and rat brain tissue sections.

Research Category
Neuroscience

Calidad

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected Neurogranin in rat brain tissue lysate.

Descripción de destino

~15 kDa observed; 7.62 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Forma física

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.