LC/MS Analysis of Nicotine and Metabolites on Kromasil® Eternity™ C18 (Analyte Set 1)
Materials
analytical column
Product No.
Description
Pricing
Kromasil® Eternity™ C18 HPLC Column
2.5 μm particle size, pore size 100 Å, L × I.D. 100 mm × 2.1 mmstandard
Product No.
Description
Pricing
(+)-Anabasine hydrochloride solution
1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®S(−)-Nicotine solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®(S)-N-Nitrosoanabasine (NAB) solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®(R,S)-Norcotinine solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®CONDITIONS
column
Kromasil Eternity C18, 10 cm x 3.0 mm I.D., 2.5 μm particles (K0897111)
mobile phase
[A] 10 mM ammonium acetate in water:methanol (95:5); [B] 10 mM ammonium acetate in water:methanol (10:90)
gradient
0% B held for 1 min; to 100% B in 8.5 min;100% B held for 1 min
flow rate
0.4 mL/min
pressure
332 psi (23 bar)
column temp.
45 °C
detector
MS, ESI(+) TOF, EIC
injection
5 μL
sample
100 ng/mL in mobile phase A
Description
Analysis Note
Nicotine and metabolites are shown here separated on a Kromasil Eternity C18 column. High purity LC/MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.
Legal Information
Eternity is a trademark of EKA Chemicals AB
Kromasil is a registered trademark of EKA Chemicals AB