HPLC Analysis of Steroids on Ascentis® Express C18 (10 cm x 2.1 mm I.D.)
Materials
analytical column
Product No.
Description
Pricing
standard
Product No.
Description
Pricing
Epitestosterone solution
1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®Methandienone solution
1.0 mg/mL in 1,2-dimethoxyethane, ampule of 1 mL, certified reference material, Cerilliant®Nandrolone solution
1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®Norethandrolone solution
1.0 mg/mL in 1,2-dimethoxyethane, ampule of 1 mL, certified reference material, Cerilliant®CONDITIONS
column
Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.7 μm particles (53823-U)
mobile phase
[A] water; [B] acetonitrile; (60:40, A:B)
flow rate
0.2 mL/min
pressure
2944 psi (203 bar)
column temp.
35 °C
detector
UV, 254 nm
injection
2 μL
sample
50 mg/L in 75:25, water:methanol
Description
Analysis Note
This application demonstrates the suitability of Ascentis Express C18 for the efficient separation of 5 steroids.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany