HPLC Analysis of Cetuximab Antibody Fragments from Papain Digestion on Zenix® SEC-150
Materials
analytical column
Product No.
Description
Pricing
Zenix® SEC-150 Gel Filtration Column, 3 μm
L × I.D. 30 cm × 7.8 mm, 3 μm particle size, stainless steelCONDITIONS
column
Zenix SEC-150, 30 cm x 7.8 mm I.D., 3 μm, 150 Å particles (Z777018)
mobile phase
20 mM sodium phosphate, pH 7.0, 150 mM NaCl
flow rate
1.0 mL/min
column temp.
25 °C
detector
UV, 280 nm
injection
10 μL
sample
Cetuximab ERBITUX®, papain digest
Description
Other Notes
Papain digestion conditions: Buffer: 5.0 mM L-Cysteine, 2.0 mM EDTA and 300 mM NaCl; Enzyme: 80 μg/mL papain; Temperature: 37 °C; Time: 30 min, 60 min, 120 min, 240 min, and 360 min.
Legal Information
Erbitux is a registered trademark of Eli Lilly and Co.
Zenix is a registered trademark of Sepax Technologies