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HPLC Analysis of Azithromycin on Ascentis® Express C18 5μm

HPLC Analysis of Azithromycin on Ascentis® Express C18 5μm application for HPLC

Materials

analytical column

Product No.
Description
Pricing

50537-U

Ascentis® Express C18, 5 μm HPLC Column

5 μm particle size, L × I.D. 15 cm × 4.6 mm

mobile phase component

Product No.
Description
Pricing

452289

Phosphoric acid

≥85 wt. % in H2O, ≥99.999% trace metals basis

standard

Product No.
Description
Pricing

75199

Azithromycin

CONDITIONS

column

Ascentis Express C18, 15 cm x 4.6 mm I.D., 5 μm particles (50537-U)

mobile phase

[A] 10 mM potassium phosphate, dibasic, pH 7.0 with phosphoric   acid; [B] methanol; (20:80, A:B)

flow rate

1.5 mL/min

pressure

2558 psi (176 bar)

column temp.

45 °C

detector

UV, 210 nm

injection

10 μL

Description

Analysis Note

A simple high-performance liquid chromatography (HPLC) method was developed for the determination of azithromycin in raw materials and pharmaceutical formulations without any pretreatment or derivatization step.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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