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  • Fast targeted analysis of 132 acidic and neutral drugs and poisons in whole blood using LC-MS/MS.

Fast targeted analysis of 132 acidic and neutral drugs and poisons in whole blood using LC-MS/MS.

Forensic science international (2014-05-06)
Matthew Di Rago, Eva Saar, Luke N Rodda, Sophie Turfus, Alex Kotsos, Dimitri Gerostamoulos, Olaf H Drummer
ABSTRACT

The aim of this study was to develop an LC-MS/MS based screening technique that covers a broad range of acidic and neutral drugs and poisons by combining a small sample volume and efficient extraction technique with simple automated data processing. After protein precipitation of 100μL of whole blood, 132 common acidic and neutral drugs and poisons including non-steroidal anti-inflammatory drugs, barbiturates, anticonvulsants, antidiabetics, muscle relaxants, diuretics and superwarfarin rodenticides (47 quantitated, 85 reported as detected) were separated using a Shimadzu Prominence HPLC system with a C18 separation column (Kinetex XB-C18, 4.6mm×150mm, 5μm), using gradient elution with a mobile phase of 25mM ammonium acetate buffer (pH 7.5)/acetonitrile. The drugs were detected using an ABSciex(®) API 2000 LC-MS/MS system (ESI+ and -, MRM mode, two transitions per analyte). The method was fully validated in accordance with international guidelines. Quantification data obtained using one-point calibration compared favorably to that using multiple calibrants. The presented LC-MS/MS assay has proven to be applicable for determination of the analytes in blood. The fast and reliable extraction method combined with automated processing gives the opportunity for high throughput and fast turnaround times for forensic and clinical toxicology.

MATERIALS
Product Number
Brand
Product Description

Supelco
Sodium hydroxide concentrate, 0.1 M NaOH in water (0.1N), Eluent concentrate for IC
Supelco
Prednisolone, Pharmaceutical Secondary Standard; Certified Reference Material
Supelco
Metronidazole, Pharmaceutical Secondary Standard; Certified Reference Material
Supelco
Valproic acid, Pharmaceutical Secondary Standard; Certified Reference Material
Supelco
Trimethoprim, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Methanol, NMR reference standard
Supelco
Difenacoum, PESTANAL®, analytical standard
Salicylic acid, European Pharmacopoeia (EP) Reference Standard
Supelco
Salicylic acid, 1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®
Sigma-Aldrich
Ammonia-14N, 99.99 atom % 14N
USP
Chlorothiazide, United States Pharmacopeia (USP) Reference Standard
Prednisolone for system suitability, European Pharmacopoeia (EP) Reference Standard
Papaverine hydrochloride, European Pharmacopoeia (EP) Reference Standard
Prednisolone, European Pharmacopoeia (EP) Reference Standard
USP
Trimethoprim, United States Pharmacopeia (USP) Reference Standard
Supelco
Chlorothiazide, Pharmaceutical Secondary Standard; Certified Reference Material
Supelco
Papaverine hydrochloride, Pharmaceutical Secondary Standard; Certified Reference Material
Paracetamol, European Pharmacopoeia (EP) Reference Standard
Supelco
Metronidazole, VETRANAL®, analytical standard
Sigma-Aldrich
Ammonia, puriss., anhydrous, ≥99.95%
Supelco
Methanol, analytical standard
Sigma-Aldrich
Salicylic acid, meets analytical specification of Ph. Eur., BP, USP, 99.5-100.5% (calc. to the dried substance)
Supelco
Prednisolone, VETRANAL®, analytical standard
Supelco
Trimethoprim, VETRANAL®, analytical standard
Sigma-Aldrich
Trimethoprim, ≥99.0% (HPLC)
Sigma-Aldrich
Sodium hydroxide solution, BioUltra, for molecular biology, 10 M in H2O
Sigma-Aldrich
Sodium hydroxide, BioUltra, for luminescence, ≥98.0% (T), pellets
Sigma-Aldrich
Salicylic acid, ACS reagent, ≥99.0%
Sigma-Aldrich
Salicylic acid, ≥99%, FG
Sigma-Aldrich
3-Ethyl-2,4-pentanedione, mixture of tautomers, 98%