- Habiterpenol, a novel abrogator of bleomycin-induced G2 arrest in Jurkat cells, produced by Phytohabitans suffuscus 3787_5.
Habiterpenol, a novel abrogator of bleomycin-induced G2 arrest in Jurkat cells, produced by Phytohabitans suffuscus 3787_5.
The Journal of antibiotics (2014-06-12)
Ryuji Uchida, Sayaka Yokota, Daisuke Matsuda, Atsuko Matsumoto, Susumu Iwamoto, Hideyuki Onodera, Yoko Takahashi, Hiroshi Tomoda
PMID24916893
ABSTRACT
A small molecule named habiterpenol produced by actinomycete Phytohabitans suffuscus 3787_5 was found to abrogate bleomycin-induced G2 arrest in Jurkat cells. Habiterpenol showed no cytotoxic effect on Jurkat cells even at 273 μM; however, the compound inhibited bleomycin-induced G2 arrest in Jurkat cells with an IC50 value of 3.55 μM, while it showed no effect on colchicine-induced M arrest even at 273 μM. These results indicated that habiterpenol selectively abrogated bleomycin-induced G2 arrest in Jurkat cells.
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
Chloroform-d, ≥99.8 atom % D, contains 0.5 wt. % silver foil as stabilizer, 0.03 % (v/v) TMS
Sigma-Aldrich
Chloroform-d, "100%", 99.96 atom % D, contains 0.5 wt. % silver wire as stabilizer
Sigma-Aldrich
Thiazolyl Blue Tetrazolium Bromide, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥97.5% (HPLC)
Colchicine, (European Pharmacopoeia (EP) Reference Standard)