- Imaging of DNA in human and plant chromosomes by high-resolution scanning electron microscopy.
Imaging of DNA in human and plant chromosomes by high-resolution scanning electron microscopy.
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology (1995-09-01)
G Wanner, H Formanek
PMID7551552
ABSTRACT
We describe a DNA-specific staining procedure, using a blue platinum organic dye, which allows DNA imaging of chromosomes by detection of back-scattered electrons in the scanning electron microscope. DNA distribution is visualized within chromosomal details of the centromeric and satellite regions, or in interphase chromatin, with high-resolution (10-15 nm) for comparison with the corresponding secondary electron image representing DNA plus protein.
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