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  • Effect of ultraviolet light absorbers on photostabilization of azadirachtin-A in solution (part: II).

Effect of ultraviolet light absorbers on photostabilization of azadirachtin-A in solution (part: II).

Natural product research (2003-04-04)
P T Deota, P R Upadhyay, V B Valodkar
ABSTRACT

The effect of photostabilization of azadirachtin-A (Aza-A) was examined in solutions when exposed to UV radiation, in the presence of four structurally different UV absorbers namely, p-aminobenzoic acid, 2,4-dihydroxybenzophenone, 4,4'-dihydroxybenzophenone and phenyl salicylate. The percentages of Aza-A recovered from the solutions after 6 h exposed to UV radiation in the presence and absence of UV absorbers indicated that the order of stabilization of Aza-A by these absorbers was similar to that obtained in the solid phase experiments in accordance with our previous observations. It is observed that the addition of phenyl salicylate in Aza-A (in 1:1 mole ratio) provides the excellent photostabilization of Aza-A molecule in solid phase as well as in solution among the four absorbers studied.

MATERIALS
Product Number
Brand
Product Description

Supelco
Phenyl salicylate melting point standard, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Phenyl salicylate, ReagentPlus®, 99%
Sigma-Aldrich
Phenyl salicylate, ≥99%, FG
Supelco
Mettler-Toledo Calibration substance ME 30034252, Phenyl salicylate, traceable to primary standards