SAB4702130

Monoclonal Anti-CD22, purified produced in mouse

• Synonym(s): SIGLEC-2, SIGLEC2
• UNSPSC Code: 12352203

Properties

• conjugate: unconjugated
• Quality Level: 100
• antibody product type: primary antibodies
• clone: S-HCL-1, monoclonal
• form: buffered aqueous solution
• species reactivity: human
• concentration: 1 mg/ml
• technique(s): flow cytometry: suitable
• isotype: IgG2bκ
• UniProt accession no.: P20273
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... CD22(933)

Description

General description

CD22, also known as Siglec-2 (sialic acid-binding immunoglobulin-like lectin-2) is a transmembrane glycoprotein binding alpha2,6-linked sialic acid-bearing ligands. Intracellular domain of CD22 recruits protein tyrosine phosphatase SHP-1 through the immunoreceptor tyrosine-based inhibitory motifs (ITIMs), thus setting a treshold for B cell receptor-mediated activation. CD22 also regulates B-cell response by involvement in controlling the CD19/CD21-Src-family protein tyrosine kinase amplification pathway and CD40 signaling. CD22 exhibits hallmarks of clathrin-mediated endocytic pathway.

Specificity

The mouse monoclonal antibody S-HCL-1 (also known as S-HCL1) recognizes an extracellular epitope of CD22 (BL-CAM), a 130 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed in the cytoplasm of pro-B and pre-B lymphocytes, and on the surface of mature and activated B lymphocytes; it is lost on plasma cells, peripheral blood T lymphocytes, granulocytes and monocytes.

Immunogen

Whole hairy cell leukemia cells and membrane preparation

Physical form

Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Preparation Note

Purified by protein-A affinity chromatography.

Storage and Stability

Store at 2-8°C. Do not freeze.

Other Notes

The antibody solution should be gently mixed before use.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable