MOPS – EDTA – Sodium Acetate (MESA) is the most commonly used running buffer for RNA electrophoresis prior to Northern blotting procedures. Applied voltage of 5 V/cm is recommended for maximum resolution.
Application
Suitable for use when making formaldehyde-agarose gels and associated running buffer for RNA electrophoresis.
Components
1x MESA buffer contains:
40 mM MOPS
10 mM sodium acetate
1 mM EDTA (pH 8.3)
Reconstitution
Dissolve entire contents of bottle in a final volume of 1L molecular biology grade water. This produces a 10x MESA stock solution that can be further diluted as needed.
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TAE and TBE are both used as running buffers for nucleic acid electrophoresis but have some important differences. Review our recipes and video to give your application the best chance of success.
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
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