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Merck

S0389

Sigma-Aldrich

Sucrose

for molecular biology, ≥99.5% (GC)

Synonym(s):

α-D-Glc-(1→2)-β-D-Fru, α-D-Glucopyranosyl β-D-fructofuranoside, β-D-Fructofuranosyl-α-D-glucopyranoside, D(+)-Saccharose, Sugar

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About This Item

Empirical Formula (Hill Notation):
C12H22O11
CAS Number:
Molecular Weight:
342.30
Beilstein:
90825
EC Number:
MDL number:
UNSPSC Code:
12352201
PubChem Substance ID:
NACRES:
NA.31

biological source

sugar cane

Quality Level

grade

for molecular biology

Assay

≥99.5% (GC)

form

crystals

technique(s)

HPLC: suitable
cryopreservation: suitable
electron microscopy: suitable
enzyme immunoassay: suitable
gas chromatography (GC): suitable
immunohistochemistry: suitable

impurities

≤0.1% free glucose

color

white

useful pH range

5.5-7 (25 °C, 342 g/L)

mp

185-187 °C (lit.)

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1 of 4

This Item
PHR10011.07651S1888
Sucrose for molecular biology, ≥99.5% (GC)

S0389

Sucrose

Sucrose Pharmaceutical Secondary Standard; Certified Reference Material

PHR1001

Sucrose

Sucrose suitable for microbiology

1.07651

Sucrose

Sucrose ≥99.5% (GC), BioReagent, suitable for cell culture, suitable for insect cell culture

S1888

Sucrose

grade

for molecular biology

grade

certified reference material, pharmaceutical secondary standard

grade

-

grade

-

biological source

sugar cane

biological source

-

biological source

-

biological source

sugar cane

technique(s)

HPLC: suitable, electron microscopy: suitable, gas chromatography (GC): suitable, cryopreservation: suitable, immunohistochemistry: suitable, enzyme immunoassay: suitable

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

technique(s)

-

technique(s)

cell culture | insect: suitable, cell culture | mammalian: suitable

Quality Level

300

Quality Level

300

Quality Level

200

Quality Level

300

solubility

H2O: soluble 50 g + 50 mL

solubility

-

solubility

-

solubility

H2O: 500 mg/mL

General description

Sucrose, a disaccharide composed of glucose and fructose, serves as an energy source in various biological processes. In cell biology, sucrose acts as a cryoprotectant, preserving cell viability during freezing and thawing. It is also added as a supplement in culture media, aiding in maintaining osmotic balance and enhancing cell survival.In molecular biology, sucrose is crucial in gradient solutions for DNA and RNA purification and in density centrifugation for organelle separation.

Application

Sucrose has been used:
  • to perform cryoprotection for kidney tissues
  • as a component in the solid germination medium to germinate pollen grains
  • in the triphenyl-tetrazolium chloride (TTC) test to check the viability of pollen grains
  • in the cryoprotection of fixed brain tissues for immunohistochemistry

Biochem/physiol Actions

Sucrose is used as a transport and storage molecule in most plants due to its limited chemical reactivity. It can be used to prepare density gradients for the separation of cell/organelle. Sucrose can be used in the plant, insect, and bacterial culture media as a supplement. In addition, sucrose can also be used in several enzymatic assays.

Features and Benefits

  • Ideal for Molecular Biology, Cell Biology and Biochemical research
  • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
  • Tested for DNase, RNase, and proteases
  • Molecular biology grade sucrose, sourced from sugar cane

Other Notes

For additional information on our range of Biochemicals, please complete this form.
To gain a comprehensive understanding of our extensive range of Disaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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