lncRNA GAS5 enhances G1 cell cycle arrest via binding to YBX1 to regulate p21 expression in stomach cancer.

lncRNA GAS5 enhances G1 cell cycle arrest via binding to YBX1 to regulate p21 expression in stomach cancer.

Scientific reports (2015-05-12)

Yongchao Liu, Jing Zhao, Wenhong Zhang, Jun Gan, Chengen Hu, Guangjian Huang, Ying Zhang

ABSTRACT

Long non-coding RNAs (lncRNAs), which have evolved as important gene expression modulators, are involved in human malignancies. The down-regulation of lncRNA growth arrest specific transcript 5 (GAS5) has been reported in several cancers, however, the underlying mechanism of lncRNA GAS5 in stomach cancer is poorly understood. In this study, we found that lncRNA GAS5 had lower expression in stomach cancer tissues than the normal counterparts. lncRNA GAS5 was shown to interact with Y-box binding protein 1 (YBX1), and lncRNA GAS5 knockdown was shown to accelerate YBX1 protein turnover without affecting YBX1 transcription. lncRNA GAS5 down-regulation reduced the YBX1 protein level, which decreased YBX1-transactivated p21 expression and abolished G1 phase cell cycle arrest in stomach cancer. These results delineate a novel mechanism of lncRNA GAS5 in suppressing stomach carcinogenesis, and the lncRNA GAS5/YBX1/p21 pathway we discovered may provide useful targets for developing lncRNA-based therapies for stomach cancer.

MATERIALS

Product Number

Brand

Product Description

11685597910

Roche

Biotin RNA Labeling Mix, sufficient for 20 reactions (transcription), pkg of 40 μL, solution

17-700

Sigma-Aldrich

Magna RIP^® RNA-Binding Protein Immunoprecipitation Kit, RNA Immunoprecipitation (RIP) Kit containing all necessary reagents to perform 12 individual RNA-binding protein immunoprecipitation (RIP) reactions using protein A/G magnetic beads.

RPOLT7-RO

Roche

T7 RNA Polymerase, from Escherichia coli BL 21/pAR 1219

RPOLSP6-RO

Roche

SP6 RNA Polymerase, from Escherichia coli BL 21/pSR3