Skip to Content
Merck

Dye-ligand affinity chromatography.

Methods in molecular biology (Clifton, N.J.) (1992-01-01)
S J Burton
ABSTRACT

Dye-ligand affinity chromatography is performed with an adsorbent consisting of a solid support matrix to which a dye has been cova-lently bonded. The protein mixture is passed through a packed bed of adsorbent that selectively binds proteins that are able to interact with the immobilized dye. Nonbound proteins are washed from the adsorbent bed and bound protein(s) are subsequently eluted by adjusting the composition of the eluant.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Mimetic Yellow 1 PIKSI Column, suspension
Sigma-Aldrich
Mimetic Blue 2 PIKSI Column, suspension
Sigma-Aldrich
Mimetic Green 1 Ligand Affinity Adsorbent, suspension
Sigma-Aldrich
Mimetic Orange 3 Ligand Affinity Adsorbent, suspension
Sigma-Aldrich
Mimetic Yellow 2 PIKSI Column, suspension
Sigma-Aldrich
Mimetic Blue AP Ligand Affinity Adsorbent, suspension
Sigma-Aldrich
Mimetic Orange 3 PIKSI Column, suspension
Sigma-Aldrich
Mimetic Red 2 Ligand Affinity Adsorbent, suspension
Sigma-Aldrich
Mimetic Blue AP PIKSI column, suspension
Sigma-Aldrich
Mimetic Green 1 PIKSI Column, suspension
Sigma-Aldrich
Mimetic Blue SA PIKSI Column, suspension
Sigma-Aldrich
Mimetic Orange 1 PIKSI Column, suspension
Sigma-Aldrich
Mimetic Red 2 PIKSI column, suspension
Sigma-Aldrich
Mimetic Red 3 Ligand Affinity Adsorbent, suspension
Sigma-Aldrich
Mimetic Orange 1 Ligand Affinity Adsorbent, suspension