94740
Vanadyl ribonucleoside complexes
BioReagent, for molecular biology
Synonym(s):
Ribonucleoside vanadyl complexes, VRC
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.52
Recommended Products
grade
for molecular biology
product line
BioReagent
form
powder
suitability
in accordance for ribonuclease inhibition test
shipped in
wet ice
storage temp.
−20°C
General description
Vanadyl ribonucleoside complexes are low molecular weight inhibitors of ribonucleases.
Application
Vanadyl ribonucleoside complexes (VDR) are transition state analogs that bind to active sites of many RNases, inhibiting their activity. Because the RNases are not covalently modified by the complexes, VDR must be used at every stage of RNA extraction and purification. However, VDR also inhibit RNA polymerases and in vitro translation and therefore must be removed from final preparation of RNA.
Vanadyl ribonucleoside complexes has been used in hybridization mixture for RNA in situ hybridization.
Biochem/physiol Actions
The vanadyl ribonucleoside complex has the ability to block the formation of ribosomal subunit in Staphylococcus aureus. The complex exhibits anti-microbial activity These complexes also serve as powerful inhibitors of several enzymes with ribonucleolytic activity.
related product
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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We have developed a simple, rapid, and reliable protocol for the small-scale purification of DNA and RNA from, e.g., human serum and urine. The method is based on the lysing and nuclease-inactivating properties of the chaotropic agent guanidinium thiocyanate together
Inhibitors of RNases
Sambrook, J. and Russell, D.W. et al.
Molecular Cloning: A Laboratory Manual, 1, 7-7 (2001)
Effect of ribonucleoside-vanadyl complexes on enzyme-catalyzed reactions central to recombinant DNA technology
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