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Key Documents

AB1426

Sigma-Aldrich

Anti-Uncoupling Protein 1 Antibody

Chemicon®, from rabbit

Synonym(s):

UCP1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human, rat, mouse

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
western blot: suitable

NCBI accession no.

NM_021833.3

UniProt accession no.

P25874

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... UCP1(7350)

Related Categories

Specificity

Reactive with mouse, rat, and human UCP1. Reactivity confirmed by western blot of purified UCP from brown adipose tissue. No cross-reactivity with UCP2.

Immunogen

Synthetic peptide corresponding to amino acids 145-159 of mouse UCP1. (C)SHLHGIKPRYTGTYN

Application

ELISA: 1:100,000

Western blot: 1:1,000

Optimal working dilutions must be determined by the end user.
Research Category
Metabolism
Research Sub Category
Ion & Transport Channels
This Anti-Uncoupling Protein 1 Antibody is validated for use in ELISA, WB for the detection of Uncoupling Protein 1.

Physical form

Format: Purified
Protein A-purified rabbit immunoglobulin. Liquid. Buffer = 100 mM KHPO4, 140 mM NaCl, pH 7. Contains no preservatives.

Storage and Stability

Maintain at -20 in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Hongyi Zhou et al.
Journal of lipid research, 56(10), 1912-1925 (2015-08-14)
Mutations in BSCL2/SEIPIN cause Berardinelli-Seip congenital lipodystrophy type 2 (BSCL2), but the mechanisms whereby Bscl2 regulates adipose tissue function are unclear. Here, we generated adipose tissue (mature) Bscl2 knockout (Ad-mKO) mice, in which Bscl2 was specifically ablated in adipocytes of
Han Fang et al.
iScience, 24(5), 102470-102470 (2021-06-12)
Dietary methionine restriction (MR) is normally implemented using diets formulated from elemental amino acids (AA) that reduce methionine content to ∼0.17%. However, translational implementation of MR with elemental AA-based diets is intractable due to poor palatability. To solve this problem
Kana Oue et al.
The Journal of biological chemistry, 291(8), 4185-4196 (2015-12-27)
Phospholipase C-related catalytically inactive protein (PRIP) was first identified as an inositol 1,4,5-trisphosphate-binding protein, and was later found to be involved in a variety of cellular events, particularly those related to protein phosphatases. We previously reported that Prip knock-out (KO)
RIP140-targeted repression of gene expression in adipocytes.
Christian, M; Kiskinis, E; Debevec, D; Leonardsson, G; White, R; Parker, MG
Molecular and cellular biology null
Hwan Hee Lee et al.
Nature communications, 10(1), 3536-3536 (2019-08-08)
Tonicity-responsive enhancer binding protein (TonEBP or NFAT5) is a regulator of cellular adaptation to hypertonicity, macrophage activation and T-cell development. Here we report that TonEBP is an epigenetic regulator of thermogenesis and obesity. In mouse subcutaneous adipocytes, TonEBP expression increases > 50-fold
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