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05-118

Sigma-Aldrich

Anti-FGF-2/basic FGF Antibody, clone bFM-2

clone bFM-2, Upstate®, from mouse

Synonym(s):

Basic fibroblast growth factor, basic fibroblast growth factor bFGF, fibroblast growth factor 2, fibroblast growth factor 2 (basic), heparin-binding growth factor 2

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

bFM-2, monoclonal

species reactivity

mouse, bovine, human, rat

manufacturer/tradename

Upstate®

technique(s)

immunohistochemistry: suitable
radioimmunoassay: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

Related Categories

General description

FGF basic (FGF2) is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. FGF2 is involved in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. FGF2 mRNA contains multiple polyadenylation sites, and is alternatively translated from AUG and non-AUG (CUG) initiation codons resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.
This antibody can cross-react with native and heat-inactivated basic FGF but will not neutralize biological activity. Upstate recommends anti-FGF-2/basic FGF, neutralizing (Catalog #05-117) for neutralizing activity.

Specificity

This antibody detects FGF-2/basic FGF.
This highly specific antibody does not cross-react with bovine acidic FGF (FGF-1, HBGF-1).

Important Note: This antibody can cross-react with native and heat-inactivated basic FGF but will not neutralize biological activity (Uteza, Y., 1999). Upstate recommends anti-FGF-2/basic FGF, clone bFM-1 (neutralizing) (Catalog # 05-117) for neutralizing activity.

Immunogen

Purified bovine brain basic FGF (FGF-2, HBGF-2). Clone bFM-2.

Application

Detect FGF-2/basic FGF using this Anti-FGF-2/basic FGF Antibody, clone bFM-2 validated for use in IH, RIA & WB.
Immunohistochemistry:
Previous lots of this antibody have been shown to immunostain frozen brain sections fixed with ice-cold, ethanol:acetic acid [95:5]. This antibody has also been used at 10 μg/mL to stain paraffin-embedded sections (Matsuzaki, K., 1989).
Research Category
Signaling
Research Sub Category
Growth Factors & Receptors

Quality

Evaluated on basic FGF (Catalog #01-114).

Western Blot Analysis:
0.5-2 μg/mL of this antibody detected 100 ng FGF-2/basic FGF, carrier-free (Catalog # 01-114).

Target description

17.5 kDa

Physical form

Ammonium sulfate precipitation and DEAE-cellulose chromatography
Format: Purified
Purified mouse monoclonal IgG1κ in buffer containing PBS, pH 7.4, purified by ammonium sulfate precipitation and DEAE Sephacryl chromatography. Frozen solution.

Storage and Stability

Stable for 1 year at -20ºC from date of receipt.

Analysis Note

Control
Fetal liver or kidney tissue.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Immunolocalization and temporal distribution of cytokine expression during the development of vein graft intimal hyperplasia in an experimental model.
P L Faries, M L Marin, F J Veith, J A Ramirez, W D Suggs, R E Parsons, L A Sanchez, R T Lyon
Journal of Vascular Surgery null
Astrid Parenti et al.
American journal of physiology. Heart and circulatory physiology, 286(5), H1978-H1984 (2003-12-25)
Vessel wall remodeling is a complex phenomenon in which the loss of differentiation of vascular smooth muscle cells (VSMCs) occurs. We investigated the role of rat macrophage chemoattractant protein (MCP)-1 on rat VSMC proliferation and migration to identify the mechanism(s)
Immunohistochemical localization of cytokines in nevi.
M G Fleming, S F Howe, A G Candel
The American Journal of Dermatopathology null
Y Uteza et al.
Proceedings of the National Academy of Sciences of the United States of America, 96(6), 3126-3131 (1999-03-17)
We developed an experimental approach with genetically engineered and encapsulated mouse NIH 3T3 fibroblasts to delay the progressive degeneration of photoreceptor cells in dark-eyed Royal College of Surgeons rats. These xenogeneic fibroblasts can survive in 1. 5-mm-long microcapsules made of
Immunolocalization of acidic and basic fibroblast growth factors in porcine uterine and conceptus tissues
Gupta, A., et al
Biology of Reproduction, 56, 1527-1536 (1997)

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