Skip to Content
Merck
  • Influence of rutin, FeSO4, Tween 80, aspartate and complex vitamins on synthesis of fungal exopolysaccharide.

Influence of rutin, FeSO4, Tween 80, aspartate and complex vitamins on synthesis of fungal exopolysaccharide.

Carbohydrate polymers (2013-02-13)
Xiao-Kui Ma, Hong Zhang, Hala Fam
ABSTRACT

The influence of several components on exopolysaccharide (EPS) production and antioxidative activity (TEAC, Trolox-Equivalent Antioxidant Capacity) as well as their effects on the morphological development and cell viability of Phellinus sp. P0988 was determined. Rutin, FeSO(4), Tween 80 and complex vitamins were found to impose a stronger influence on EPS production and TEAC compared to their effects on the mycelia growth of Phellinus sp. P0988. The relative effects of these components on EPS activity were found to be different from that on EPS yield. Rutin and aspartate significantly affected EPS TEAC (P<0.05), while FeSO(4) and Tween 80 significantly influenced EPS production (P<0.05). These results yielded the optimum culture medium composition, with an EPS yield and TEAC of 6.2±0.2 g/L and 5.5±0.1 mM, respectively.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Iron(II) sulfate heptahydrate, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥99%
Sigma-Aldrich
Iron(II) sulfate heptahydrate, suitable for plant cell culture, ≥99%
Sigma-Aldrich
Iron(II) sulfate heptahydrate, ACS reagent, ≥99.0%
Sigma-Aldrich
Iron(II) sulfate heptahydrate, puriss., meets analytical specification of Ph. Eur., BP, USP, FCC, 99.5-104.5% (manganometric)
Sigma-Aldrich
Iron(II) sulfate heptahydrate, ReagentPlus®, ≥99%