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  • The poly(C)-binding protein Pcbp2 is essential for CD4+ T cell activation and proliferation.

The poly(C)-binding protein Pcbp2 is essential for CD4+ T cell activation and proliferation.

iScience (2023-01-13)
Massimo Martinelli, Gabrielle Aguilar, David S M Lee, Andrew Kromer, Nhu Nguyen, Benjamin J Wilkins, Tatiana Akimova, Ulf H Beier, Louis R Ghanem
ABSTRACT

The RNA-binding protein Pcbp2 is widely expressed in the innate and adaptive immune systems and is essential for mouse development. To determine whether Pcbp2 is required for CD4+ T cell development and function, we derived mice with conditional Pcbp2 deletion in CD4+ T cells and assessed their overall phenotype and proliferative responses to activating stimuli. We found that Pcbp2 is essential for T conventional cell (Tconv) proliferation, working through regulation of co-stimulatory signaling. Pcbp2 deficiency in the CD4+ lineage did not impact Treg abundance in vivo or function in vitro. In addition, our data demonstrate a clear association between Pcbp2 control of Runx1 exon 6 splicing in CD4+ T cells and a specific role for Pcbp2 in the maintenance of peripheral CD4+ lymphocyte population size. Last, we show that Pcbp2 function is required for optimal in vivo Tconv cell activation in a T cell adoptive transfer colitis model system.

MATERIALS
Product Number
Brand
Product Description

Roche
Interleukin-2, mouse (mIL-2), recombinant (E. coli)
Sigma-Aldrich
Phorbol 12-myristate 13-acetate, ≥99% (TLC), film or powder