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product name
CAR, 89072611
biological source
fish fin
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Goldfish fin tissue
Cell Line Description
The cells support the growth of grunt fin agent with CPE at 20°C and 25°C. Also susceptible to infectious pancreatic necrosis virus of trout at 28°C.
Application
Growth factors and virus studies: Infectious pancreatic necrotic virus
Culture Medium
BME (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:4 using 0.25% trypsin; 5% CO2; 25°C. Fish cell lines detach easily during transit if the culture is too young. After resuscitation split cells 1:3 to 1:4 and culture for at least one week with 1-2 media ch
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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