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3014-05A

Sigma-Aldrich

Human Carotid Artery Endothelial Cells: HCtAEC, adult

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About This Item

UNSPSC Code:
41106514

biological source

human carotid artery (normal)

Quality Level

packaging

pkg of 500,000 cells

manufacturer/tradename

Cell Applications, Inc

growth mode

Adherent

karyotype

2n = 46

morphology

Endothelial

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cardiovascular diseases

shipped in

dry ice

storage temp.

−196°C

General description

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

HCtAEC has been used to elucidate molecular mechanisms of cerebral aneurism caused by haemodynamic (sheer) stress and inflammation, which were shown to act through PGE2-EP2 and NF-κB signaling (Aoki, 2011). Demonstrating a link between inflammation and neovascularization, serum amyloid A, a biomarker of inflammation, increased expression of TNF, F3 factor, NF-κB and VEGF leading to activated migration, wound healing and tube formation responses in HCtAEC; these pro-angiogenic activities could be prevented by pretreatment with the multi-angiokinase receptor inhibitor BIBF1120 (Cai, 2013). Additionally, CD40-CD154 signaling between endothelial cells and T cells leads to a stress response in endothelial cells via activation of NF-κB and MAPK/SAPK pathways and induces down-regulation of APLN, while at the same time activating viral immune surveillance system involving TLR3, IFIH1, RIG-I, and RNASEL (Pluvinet, 2008).

Also, HCtAEC, along with human aortic (HAOEC), brachiocephalic artery (HBcAEC), coronary artery (HCAEC) and subclavian artery (HScAEC) have been used to demonstrate that not only blood vessels from different tissues are highly heterogeneous, they also interact differently with leukocytes during the inflammation response (Scott, 2013). The authors further showed that differential N-glycosylation of commonly expressed vascular adhesion molecules may be responsible for this heterogeneity, as well as for modulation of signaling under resting and activated inflammatory conditions. This also explains why specific vascular beds may be more or less susceptible to particular diseases or stimuli. Importantly, if cells from different sources were used, these results could not be convincingly validated due to a number of uncontrolled variables, such as age, race, genetic variability or life style choices of the donors.

Because of the complex heterogeneity that exists not only between different donors, but even between different vascular beds in the same individual, it would be prudent to confirm any new findings on primary cell lots coming from several different origins.

Cell Line Origin

Artery

Application

neovascularization, cell migration, wound healing, tube formation, angiogenesis, signal tranduction, monocyte chemotactic factor production, cell adhesion

Components

Basal Medium containing 10% FBS & 10% DMSO

Preparation Note

  • 2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
  • Can be cultured at least 10 doublings

Subculture Routine

Please refer to the HCtAEC Culture Protocol.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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