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14-697

Sigma-Aldrich

Histone H4 Protein, human recombinant, 1 mg

Recombinant human histone H4 produced in E. coli, purified by FPLC. For use in Enzyme Assays & Western Blotting.

Synonym(s):

H4, Histone H4

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About This Item

UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.32

biological source

human

Quality Level

recombinant

expressed in E. coli

mol wt

Mw 11 kDa

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

General description

Recombinant human histone H4 produced in E. coli.

Quality

routinely evaluated as a substrate for in vitro enzymatic reactions.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Expression and purification of recombinant human histones.
Tanaka, Yoshinori, et al.
Methods, 33, 3-11 (2004)
Characterization of nucleosome core particles containing histone proteins made in bacteria.
Luger, K, et al.
Journal of Molecular Biology, 272, 301-311 (1997)
Matthew V Holt et al.
Current protocols, 1(2), e26-e26 (2021-02-04)
Histones are the primary protein component of chromatin and are involved in virtually all DNA-templated processes. Histones are abundantly post-translationally modified by a variety of chromatin-modifying machinery. These post-translational modifications (PTMs) are recognized by a range of "reader" proteins, which
Expression, purification, and structural characterization of human histone H4.
Vergani, Laura, et al.
Protein Expression and Purification, 24, 420-428 (2002)
David Shechter et al.
Nature protocols, 2(6), 1445-1457 (2007-06-05)
Histone proteins are the major protein components of chromatin, the physiologically relevant form of the genome (or epigenome) in all eukaryotic cells. Chromatin is the substrate of many biological processes, such as gene regulation and transcription, replication, mitosis and apoptosis.

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