Skip to Content
Merck
All Photos(1)

Key Documents

14-1057

Sigma-Aldrich

Human native nucleosomes

Human native nucleosomes purified from HeLa cell nuclei, digested with micrococcal nuclease & purified by glycerol gradient & ion exchange chromatography.

Synonym(s):

Human Nucleosome Preparation, Native Nucleosomes

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.32

biological source

human

Quality Level

Assay

>98% (SDS-PAGE)

form

liquid

manufacturer/tradename

Upstate®

concentration

0.2 μg/μL

technique(s)

activity assay: suitable

shipped in

dry ice

General description

Human native nucleosomes purified from HeLa cell nuclei, digested with micrococcal nuclease, and purified by glycerol gradient and ion exchange chromatography.
Nucleosomes are the basic units of chromatin, and are comprised of approximately 147 bp of DNA spooled around a central octamer of core histones, H2A, H2B, H3, and H4. The arrangement of nucleosomes in chromatin can be altered by post-translational modifications (PTM) occurring on the C- and N-terminal tails of the core histones. Histone modifications can either provide or hinder access of key transcriptional complexes to DNA sequences and can regulate the expression of genes. These effects can be deciphered by applications such as chromatin immunoprecipiation (ChIP), which requires high quality chromatin, and essential tools such as histone proteins and appropriate enzymes, to assemble chromatin in vitro.
Product Source: Human

Application

Research Category
Epigenetics & Nuclear Function
Suitable for in vitro chromatin assembly and enzyme activity assays

Physical form

20 μg of mononucleosomes in buffer containing 20 mM Tris-Cl pH 7.9, 1 mM EDTA, 0.5 mM PMSF,1 mM DTT, and 50% glycerol.

Storage and Stability

Stable at -80ºC for 1 year from date of receipt.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service