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07-450

Sigma-Aldrich

Anti-monomethyl-Histone H3 (Lys9) Antibody

Upstate®, from rabbit

Synonym(s):

H3K9me1, Histone H3 (mono methyl K9), H3 histone family, member T, histone 3, H3, histone cluster 3, H3

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

chicken, mouse, human

manufacturer/tradename

Upstate®

technique(s)

dot blot: suitable
immunocytochemistry: suitable
inhibition assay: suitable (peptide)
western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

monomethylation (Lys9)

Gene Information

human ... HIST1H3F(8968)

Related Categories

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Specificity

Broad species cross-reactivity is expected based on sequence homology.
Recognizes monomethylated histone H3 (Lys9), Mr ~17 kDa.

Immunogen

Epitope: Lys9
KLH-conjugated, synthetic 2X-branched peptide containing the sequence …ARmeKST… in which meK corresponds to monomethyl-lysine at residue 9 of human histone H3.

Application

Anti-monomethyl-Histone H3 (Lys9) Antibody is a Rabbit Polyclonal Antibody for detection of Histone H3 monomethylated on lysine 9 (H3K9me1). This purified antibody is specificity verified by dot blot (DB), published in peer reviewed journals, and has been validated in ICC, PIA & WB.
Peptide Competition:
Specificity confirmed by the ability of 10 μM of the immunizing peptide to abolish detection of histone H3 in immunoblot analysis of HeLa acid extracts (Figure B, Lane 2). No signal reductions were detected with preincubation with 10 μM di- or trimethyl-lysine 9 or di- or trimethyl-lysine 27 modified peptides. Some signal reduction detected after preincubation with 10 μM monomethyl-lysine 27 modified peptide (Figure B, Lane 5, page 2).

Immunocytochemistry & Dot Blot: Reported by an independent laboratory1-4.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones

Quality

Routinely evaluated by Western Blot on acid extracted proteins from HeLa cells, but did not detect unmethylated recombinant Histone H3.

Western Blot Analysis:
0.1-0.2 μg/mL had detected monomethylated histone H3 in acid extracted proteins from HeLa cells, but did not detect unmethylated recombinant Histone H3 (Catalog # 14-411).

Target description

17 kDa

Physical form

Format: Purified
Protein A purified
Purified rabbit IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide and 30% glycerol.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
HeLa cell extract

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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