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07-1404

Sigma-Aldrich

Anti-GLUT-4 Antibody, C-terminus

from rabbit, purified by affinity chromatography

Synonym(s):

Solute carrier family 2, facilitated glucose transporter member 4, Glucose transporter type 4, insulin-responsive, GLUT-4

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, human

species reactivity (predicted by homology)

rat (based on 100% sequence homology)

technique(s)

immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... SLC2A4(6517)

Related Categories

General description

The protein called GLUT-4, or more correctly Solute carrier family 2, facilitated glucose transporter member 4 and encoded by the human gene SLC2A4/GLUT4 is a primary insulin regulated facilitative glucose transporter found primarily in skeletal and cardiac muscle, as well as brown and white fat but is also expressed in other tissues such as brain. In the brain GLUT4 is found exclusively in neurons. GLUT-4 is facilitative because it is not always present on the cell plasma membrane. GLUT-4 only localizes to the plasma membrane upon insulin induction. GLUT-4 is localized to the perinuclear region in resting cells. Mutations in GLUT-4 are a cause of one form of non-insulin dependent diabetes mellitus. GLUT4 function as well as its transcriptional regulation is highly conserved throughout the vertebrate species.

Specificity

Glucose transporter 4 (GLUT-4) is a protein present in rat skeletal muscle, heart and adipose tissues. GLUT-4 is highly conserved between species, and the peptide sequence selected for production of this antibody is identical in rat, human (amino acids 498-510), and mouse. This antibody shows no cross-reactivity of HepG2 type transporter from rat brain or other glucose transporters.

Immunogen

Epitope: C-terminus
KLH-conjugated linear peptide corresponding to the C-terminus of human GLUT-4.

Application

Detect GLUT-4 using this Anti-GLUT-4 Antibody, C-terminus validated for use in WB, IH(P), ELISA.
Immunohistochemistry Analysis: A 1:50-2,000 dilution from a representative lot detected GLUT-4 in human cardiac myocytes and human skeletal muscle myocytes.
Research Category
Signaling
Research Sub Category
Insulin/Energy Signaling

Quality

Evaluated by Western Blotting in NIH3T3 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected GLUT-4 in 10 µg of NIH3T3 cell lysate.

Target description

~58 kDa observed

Linkage

Replaces: AB1346

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
NIH3T3 cell lysate

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Insulin and insulin-like growth factor I up-regulate GLUT4 gene expression in fetal brown adipocytes, in a phosphoinositide 3-kinase-dependent manner.
Valverde, A M, et al.
The Biochemical Journal, 337 ( Pt 3), 397-405 (1999)
Rashmi Supriya et al.
Frontiers in physiology, 7, 323-323 (2016-08-12)
Anti-cancer agent doxorubicin (DOX) has been demonstrated to worsen insulin signaling, engender muscle atrophy, trigger pro-inflammation, and induce a shift to anaerobic glycolytic metabolism in skeletal muscle. The myotoxicity of DOX in diabetic skeletal muscle remains largely unclear. This study
Dolly Al Koborssy et al.
Frontiers in neuroscience, 8, 333-333 (2014-11-18)
In the brain, glucose homeostasis of extracellular fluid is crucial to the point that systems specifically dedicated to glucose sensing are found in areas involved in energy regulation and feeding behavior. Olfaction is a major sensory modality regulating food consumption.
Ceramide mediates insulin resistance by tumor necrosis factor-alpha in brown adipocytes by maintaining Akt in an inactive dephosphorylated state.
Teruel, T, et al.
Diabetes, 50, 2563-2571 (2001)
ChREBP, a glucose-responsive transcriptional factor, enhances glucose metabolism to support biosynthesis in human cytomegalovirus-infected cells.
Yu, Y; Maguire, TG; Alwine, JC
Proceedings of the National Academy of Sciences of the USA null

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