T7575

Trypsin Singles, Proteomics Grade

• Synonym(s): Proteomics grade, Trypsin, TPCK treated
• UNSPSC Code: 12352204
• NACRES: NA.56

Properties

• grade: Proteomics Grade
• form: ready-to-use solution
• specific activity: ≥10,000 BAEE units/mg protein
• mol wt: average mol wt 23.29 kDa
• optimum pH: ~8.0
• storage temp.: 2-8°C

Description

General description

Experience all of the advantages of Proteomics Grade Trypsin in a convenient, single-use 1 μg package.

Trypsin Singles, Proteomics Grade exhibit excellent proteolytic efficiency, generating more tryptic peptides leading to greater sequence coverage of your peptide of interest. Mass spectra are significantly simplified due to the reduced number of interfering autolytic peaks and their ambiguous adducts. Proteomics Grade Trypsin has been extensively purified from porcine pancreas to enable accurate and precise cleavage on the carboxylic side of Arg and Lys residues. The enzyme has been exhaustively processed by reductive methylation to minimize autolysis and chymotryptic activity has been quenched by TPCK treatment. Further purification steps including affinity chromatography and drying from dilute acid produce a highly purified, high specific activity trypsin purposely suited for the demanding criteria of proteomics research, and designed to function in either solution or gel-based digests.

Application

Trypsin Singles, Proteomics Grade have been used for protein digestion to analyse generated peptides via MALDI-TOF MS (matrix assisted laser desorption ionization-time of flight mass spectrometry). It has been used for the digestion of proteins to analyse generated peptides via LC-MS (liquid chromatography–mass spectrometry)/MS.

Features and Benefits

• Convenient, single-use 1 μg size
• Eliminates repetitive pipetting
• Reductively methylated to minimize autolytic activity
• TPCK treated to quench chymotryptic activity
• Extensively purified

Kit Components Also Available Separately

• Trypsin Solubilization Reagent

Safety Information

• Pictograms: GHS02,GHS08,GHS07
• Signal Word: Danger
• Hazard Statements: H225,H302 + H312 + H332,H315,H319,H334,H335
• Precautionary Statements: P210 - P233 - P280 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 3 - Flammable liquids