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I6383

Sigma-Aldrich

Insulin Chain B Oxidized from bovine pancreas

≥80% (HPLC), powder

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About This Item

Empirical Formula (Hill Notation):
C157H232N40O47S2
CAS Number:
Molecular Weight:
3495.89
MDL number:
UNSPSC Code:
12352202
NACRES:
NA.77

sterility

non-sterile

Quality Level

Assay

≥80% (HPLC)

form

powder

mol wt

3496 Da by calculation (Average Mass)

UniProt accession no.

shipped in

ambient

storage temp.

−20°C

InChI

1S/C157H232N40O47S2/c1-79(2)57-104(181-130(211)86(15)172-136(217)103(50-53-125(209)210)180-152(233)127(84(11)12)194-148(229)107(60-82(7)8)184-145(226)113(67-95-70-165-78-171-95)189-150(231)115(74-198)176-123(206)73-169-133(214)116(75-245(239,240)241)191-140(221)105(58-80(3)4)182-144(225)112(66-94-69-164-77-170-94)188-138(219)102(48-51-119(160)202)178-146(227)114(68-120(161)203)190-153(234)126(83(9)10)193-131(212)98(159)61-89-31-21-18-22-32-89)139(220)185-110(64-92-40-44-96(200)45-41-92)142(223)183-106(59-81(5)6)147(228)195-128(85(13)14)154(235)192-117(76-246(242,243)244)134(215)168-71-121(204)174-101(49-52-124(207)208)137(218)177-99(38-29-55-166-157(162)163)132(213)167-72-122(205)175-108(62-90-33-23-19-24-34-90)141(222)186-109(63-91-35-25-20-26-36-91)143(224)187-111(65-93-42-46-97(201)47-43-93)149(230)196-129(88(17)199)155(236)197-56-30-39-118(197)151(232)179-100(37-27-28-54-158)135(216)173-87(16)156(237)238/h18-26,31-36,40-47,69-70,77-88,98-118,126-129,198-201H,27-30,37-39,48-68,71-76,158-159H2,1-17H3,(H2,160,202)(H2,161,203)(H,164,170)(H,165,171)(H,167,213)(H,168,215)(H,169,214)(H,172,217)(H,173,216)(H,174,204)(H,175,205)(H,176,206)(H,177,218)(H,178,227)(H,179,232)(H,180,233)(H,181,211)(H,182,225)(H,183,223)(H,184,226)(H,185,220)(H,186,222)(H,187,224)(H,188,219)(H,189,231)(H,190,234)(H,191,221)(H,192,235)(H,193,212)(H,194,229)(H,195,228)(H,196,230)(H,207,208)(H,209,210)(H,237,238)(H4,162,163,166)(H,239,240,241)(H,242,243,244)/t86-,87-,88+,98-,99-,100-,101-,102-,103-,104-,105-,106-,107-,108-,109-,110-,111-,112-,113-,114-,115-,116-,117-,118-,126-,127-,128-,129-/m0/s1

InChI key

CZJQESISDHGFTC-DQCCOYHDSA-N

Gene Information

cow ... INS(280829)

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Amino Acid Sequence

Phe-Val-Asn-Gln-His-Leu-Cys(SO3H)-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cys(SO3H)-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala

General description

Insulin is made up of two polypeptide chains (A and B) linked together by two disulfide linkages. Chain A and B are composed of 21 amino acids and 30 amino acids, respectively.

Application

Insulin Chain B Oxidized from bovine pancreas has been used:
  • as a matrix-assisted laser desorption/ionization (MALDI)-mass spectrometry (MS) calibration standard
  • in electrochemical impedance spectroscopy and quartz crystal nanobalance (EQCN) studies of insulin adsorption on platinum
  • as an internal standard to quantify insulin chain B residue in protein samples

Biochem/physiol Actions

Insulin regulates the cellular uptake, utilization, and storage of glucose, amino acids, and fatty acids. It inhibits the breakdown of glycogen, protein, and fat. The β chain is a substrate for carboxypeptidase Y.

Preparation Note

Prepared by modification of Sanger, F., et al.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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L He et al.
Journal of mass spectrometry : JMS, 34(9), 909-914 (1999-09-24)
Matrix-assisted laser desorption/ionization (MALDI) mass spectra were obtained from single particles injected directly into a time-of-flight mass spectrometer. Aerosol particles were generated at atmospheric pressure using a piezoelectric single-particle generator or a pneumatic nebulizer and introduced into the mass spectrometer
Electrochemical impedance spectroscopy and quartz crystal nanobalance (EQCN) studies of insulin adsorption on Pt
Wright JEI, et al.
Journal of Electroanalytical Chemistry, 564, 185-197 (2004)
Johansen, J.T., et al.
Carlsberg Research Communications, 41, 1-1 (1976)
Epitope mapping by a combination of epitope excision and MALDI-MS.
C E Parker et al.
Methods in molecular biology (Clifton, N.J.), 146, 185-201 (2000-08-19)
Fractionation of oxidized insulin.
F Sanger
The Biochemical journal, 44(1), 126-128 (1949-01-01)

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