71196

Sodium acetate solution

BioUltra, for molecular biology, ~3 M in H₂O

• Empirical Formula (Hill Notation): C₂H₃NaO₂
• CAS Number: 127-09-3
• Molecular Weight: 82.03
• Beilstein: 3595639
• MDL number: MFCD00012459
• UNSPSC Code: 12161700
• eCl@ss: 39021906
• PubChem Substance ID: 57652229
• NACRES: NA.21

Properties

• grade: for molecular biology
• product line: BioUltra
• form: liquid
• concentration: ~3 M in H₂O
• technique(s): precipitation assay: suitable
• impurities: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
• pH: 7.0-8.0 (25 °C, 3 M in H₂O)
• anion traces: chloride (Cl^-): ≤10 mg/kg; phosphate (PO₄^3-): ≤5 mg/kg; sulfate (SO₄^2-): ≤20 mg/kg
• cation traces: Al: ≤1 mg/kg; As: ≤0.1 mg/kg; Ba: ≤1 mg/kg; Bi: ≤1 mg/kg; Ca: ≤5 mg/kg; Cd: ≤1 mg/kg; Co: ≤1 mg/kg; Cr: ≤1 mg/kg; Cu: ≤1 mg/kg; Fe: ≤1 mg/kg; K: ≤50 mg/kg; Li: ≤1 mg/kg; Mg: ≤1 mg/kg; Mn: ≤1 mg/kg; Mo: ≤1 mg/kg; Ni: ≤1 mg/kg; Pb: ≤1 mg/kg; Sr: ≤1 mg/kg; Zn: ≤1 mg/kg
• λ: 3 M in H₂O
• UV absorption: λ: 260 nm A_max: ≤0.030; λ: 280 nm A_max: ≤0.024
• SMILES string: [Na+].CC([O-])=O
• InChI: 1S/C2H4O2.Na/c1-2(3)4;/h1H3,(H,3,4);/q;+1/p-1
• InChI key: VMHLLURERBWHNL-UHFFFAOYSA-M

Description

Application

Sodium acetate solution has been used:

• in DNA extraction
• in reverse crosslinking and DNA purification
• to precipitate Gata6 (GATA binding protein 6) cDNA as a part of high-purity plasmid preparation

Biochem/physiol Actions

Sodium acetate serves as a fixed base. It is useful to rectify acidosis among acidotic patients. Orginally, sodium acetate is used as dialysate for hemodialysis, but is known to cause cardiovascular instability.

Other Notes

Basic method of DNA precipitation with ethanol

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves