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The effect of heat stress on gene expression and synthesis of heat-shock and milk proteins in bovine mammary epithelial cells.

Animal science journal = Nihon chikusan Gakkaiho (2015-10-16)
Han Hu, Yangdong Zhang, Nan Zheng, Jianbo Cheng, Jiaqi Wang
RESUMEN

In this study, bovine mammary epithelial cells were used to study stress responses after cells were exposed to 42°C for 0.5, 1, 3, 5, 8 or 12 h, and 38°C as control. The transcription of the genes (HSP27, HSP70 and HSP90) of heat shock protein (Hsp) was significantly enhanced under heat stress (HS). The peak transcription of HSP70 was 14 times the control at 1 h. Expression of proteins Hsp27 and Hsp70 was gradually increased under HS, with rapid deposition of Hsp70 in epithelial cells. The major milk protein genes of β-casein (CSN2) and butyrophilin (BTN1A1) were down-regulated and the synthesis of total caseins was decreased. After the cells were under HS (42°C) for 1 or 5 h, the cells were cultured at 38°C for 1, 6, 12 or 24 h for recovery. When the cells were cultured at 38°C for 24 h after HS for 1 h, the transcription of HSP70, HSP90, CSN2 and BTN reached normal levels. Our results suggest that HS initiated Hsp synthesis and decreased the milk protein synthesis. Hsp70 is extremely sensitive to HS and mainly responsible for mammary cell protection from HS.

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Sigma-Aldrich
Epidermal Growth Factor from murine submaxillary gland, EGF, suitable for cell culture
Sigma-Aldrich
holo-Transferrin bovine, Iron-saturated, BioReagent, suitable for cell culture