59140-U
SUPELCOSIL™ LC-ABZ (5 µm) HPLC Columns
L × I.D. 15 cm × 4.6 mm, HPLC Column
Sinónimos:
Amino-Benzene-Zone HPLC Column
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About This Item
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product name
Columna de HPLC SUPELCOSIL™ LC-ABZ, 5 μm particle size, L × I.D. 15 cm × 4.6 mm
material
stainless steel column
Quality Level
agency
suitable for USP L60
product line
SUPELCOSIL™
feature
endcapped
manufacturer/tradename
SUPELCOSIL™
packaging
1 ea of
extent of labeling
12.0% carbon loading
parameter
≤70 °C temp. limit
400 bar pressure (5801 psi)
technique(s)
HPLC: suitable
L × I.D.
15 cm × 4.6 mm
surface area
170 m2/g
surface coverage
surface coverage 3.4 μmol/m2
matrix
silica gel, spherical particle platform
fully porous particle
matrix active group
amide, alkyl phase
particle size
5 μm
pore size
120 Å
pH range
2-7.5
application(s)
food and beverages
separation technique
reversed phase
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General description
SUPELCOSIL LC-ABZ phase has a unique deactivation technology which provides excellent reversed phase performance for basic compounds, as well as those that are acidic, polar neutral, and non-polar. Silanol suppressing additives and techniques such as competing amines, high ionic strength, and pH extremes are typically not required to obtain good peak shape on the LC-ABZ phase.
Application
- Validation of a high-performance liquid chromatography method for the assay of and determination of related organic impurities in nevirapine drug substance.: This paper validates a method using the SUPELCOSIL™ LC-ABZ HPLC Column for assessing purity and impurities in HIV medication, showcasing the column′s high specificity and reproducibility (Li et al., 2000).
- A comparative study of the reversed-phase HPLC retention behaviour of S-adenosyl-L-methionine and its related metabolites on Hypersil ODS and Supelcosil LC-ABZ stationary phases.: This comparative study provides insights into the retention behaviors of bioactive compounds on SUPELCOSIL™ LC-ABZ, affirming its versatility and robust performance in complex biochemical separations (Valkó et al., 1993).
Features and Benefits
• For acids, bases, zwitterions
• Increased polar retention relative to standard reversed-phases
• Unique selectivity
• Silanol deactivation
• Peak shape, efficiency, and retention are comparable to C8 phases for non-polar analytes
• Increased polar retention relative to standard reversed-phases
• Unique selectivity
• Silanol deactivation
• Peak shape, efficiency, and retention are comparable to C8 phases for non-polar analytes
Legal Information
SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC
Application
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Descripción
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guard cartridge
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related product
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Storage Class
11 - Combustible Solids
wgk_germany
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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J G Ufkes et al.
Pharmacy world & science : PWS, 20(2), 83-87 (1998-05-19)
To study the pharmacokinetics of dextromoramide in long-term opiate addicts on methadone maintenance therapy (MMT) a reverse-phase HPLC technique was developed to monitor dextromoramide and methadone concentrations in plasma simultaneously. After liquid-liquid extraction from plasma, dextromoramide and methadone were determined
Neil C Dias et al.
The Analyst, 128(5), 427-433 (2003-06-07)
The solvation parameter model is used to identify suitable chromatographic models for estimating the octanol-water partition coefficient for neutral compounds of varied structure by reversed-phase liquid chromatography. The stationary phase Supelcosil LC-ABZ with methanol-water mobile phases affords a series of
D Korakas et al.
Journal of pharmaceutical and biomedical analysis, 12(9), 1141-1145 (1994-09-01)
A series of biologically active peptides and their conjugates with lipidic amino acids were investigated by systematic change of the mobile phase composition using traditional octadecylsilica stationary phase and the newly developed Supelcosil LC-ABZ column. The mobile phases contained various
Murali Pendela et al.
Journal of separation science, 32(20), 3418-3424 (2009-09-19)
Streptomycin (SM) is composed of streptidine, streptose and N-methyl glucosamine sugar moieties. For the determination of SM and its related substances, an ion-pair LC-UV detection method using a Supelcosil LC-ABZ column was developed previously. While analyzing commercial samples, several unknown
Q C Li et al.
Journal of chromatographic science, 38(6), 246-254 (2000-07-13)
Nevirapine (Viramune), a dipyridiodiazepinone, is a potent and highly specific nonnucleoside inhibitor of HIV-1 reverse transcriptase. This paper describes the validation of a specific, sensitive, and stability-indicating high-performance liquid chromatography method for the assay and determination of related organic impurities
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