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R8508

Sigma-Aldrich

Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae)

buffered aqueous solution

Sinónimos:

Transfer RNA, tRNA

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About This Item

Número de CAS:
9014-25-9
MDL number:
MFCD00132200
UNSPSC Code:
12352106
NACRES:
NA.55

grade

for molecular biology

Quality Level

200

form

buffered aqueous solution

concentration

9-11 mg/mL

foreign activity

DNase, Nickase, none detected

shipped in

dry ice

storage temp.

−20°C

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General description

Transfer RNA (tRNA) is isolated from baker′s yeast.
Transfer RNA (tRNA) is a short nucleotide RNA chain, which has an L-shaped structure.

Application

Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae) has been used to precipitate mRNA of denuded oocytes and for reverse transcription. It has also been used to seal magnetic beads in RNA pull-down assay of nasopharyngeal carcinoma (NPC) cells.
Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae) is suitable for use as a carrier in nucleic acid purification and precipitation.

Biochem/physiol Actions

Transfer RNA (tRNA) acts as an ‘adaptor′ molecule that translates the three-nucleotide codon sequence in the mRNA to the corresponding amino acid of that codon. It determines the genetic code by linking amino acids and nucleic acids. tRNA is involved in viral replication, bacterial cell wall biosynthesis, cell stress response, and animal behavior control. Aberrations in tRNA molecules are associated with several human diseases.

Components

tRNA is provided in a solution in 10 mM Tris HCl (pH 7.4) in 1 mM EDTA.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Patrick Lonergan et al.
Molecular reproduction and development, 66(3), 297-305 (2003-09-23)
In the cyclic cow, final maturation of the ovulatory follicle is initiated by the preovulatory luteinizing hormone (LH) surge. During the subsequent 24 hr period, the oocyte nucleus undergoes meiotic progression to metaphase II and several changes in cytoplasmic organization
Encyclopedia of Agriculture and Food Systems (2015)
Andreas M Anger et al.
Nature, 497(7447), 80-85 (2013-05-03)
Protein synthesis in all cells is carried out by macromolecular machines called ribosomes. Although the structures of prokaryotic, yeast and protist ribosomes have been determined, the more complex molecular architecture of metazoan 80S ribosomes has so far remained elusive. Here
Wolf D Hirschmann et al.
Nucleic acids research, 42(6), 4043-4055 (2014-01-22)
The budding yeast multi-K homology domain RNA-binding protein Scp160p binds to >1000 messenger RNAs (mRNAs) and polyribosomes, and its mammalian homolog vigilin binds transfer RNAs (tRNAs) and translation elongation factor EF1alpha. Despite its implication in translation, studies on Scp160p's molecular
Naohiro Terasaka et al.
Nature chemical biology, 10(7), 555-557 (2014-06-09)
The Watson-Crick base pairs between the 3'-terminal end of tRNAs and ribosomal RNA in the peptidyl transferase center are universally conserved. Here, we report that the introduction of compensatory mutations to Escherichia coli RNAs in this site leads to an
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