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P2088

Sigma-Aldrich

Peroxidasa from horseradish

Highly stabilized, essentially salt-free, lyophilized powder, 200-300 units/mg solid (using pyrogallol)

Sinónimos:

Donante:peróxido de hidrógeno oxidorreductasa, Peroxidasa de rábano picante

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About This Item

Número de CAS:
9003-99-0
Comisión internacional de enzimas:
1.11.1.7 (BRENDA, IUBMB)
EC Number:
232-668-6
MDL number:
MFCD00071339
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

horseradish

form

essentially salt-free, lyophilized powder

specific activity

200-300 units/mg solid (using pyrogallol)

mol wt

~44 kDa

absorbance ratio

RZ 2.6-3.4

storage temp.

2-8°C

InChI

1S/H2O3/c1-3-2/h1-2H

InChI key

JSPLKZUTYZBBKA-UHFFFAOYSA-N

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General description

La peroxidasa de rábano picante se aísla de las raíces de rábano picante (Amoracia rusticana) y pertenece al grupo de ferroprotoporfirina de las peroxidasas. La HRP es un polipéptido monocatenario que contiene cuatro puentes bisulfuro. Es una glucoproteína que contiene un 18 % de carbohidratos. La composición de los carbohidratos consiste en galactosa, arabinosa, xilosa, fucosa, manosa, manosamina y galactosamina dependiendo de la isoenzima específica. Su peso molecular (~44 kDa) incluye la cadena polipeptídica (33 890 dalton), la hemina más Ca2+ (~700 dalton) y los carbohidratos (~9 400 dalton). Existen al menos siete isoenzimas de la HRP. El punto isoeléctrico de las isoenzimas de la peroxidasa de rábano picante oscila entre 3,0 y 9,0.

Application

Peroxidase from horseradish has been used in a study with dehydrogenative polymers (DHP) to investigate the monolignol coupling mechanism. It has also been used to help develop a colorimetric method for microRNA (miRNA) detection by creating a horseradish peroxidase-mimicking DNAzyme.

Biochem/physiol Actions

Horseradish peroxidase has been shown to catalyze the polymerization of various substances including acetaminophen and cardanol.

Caution

Maintains activity at low pH and higher temperature.

Unit Definition

Una unidad de pirogalol formará 1,0 mg de purpurogalina a partir de pirogalol en 20 segundos a pH 6,0 a 20 °C.

Preparation Note

Stabilized by chemical protection of the primary amines.

Analysis Note

La RZ (Reinheitszahl) es la relación de absorbancia A403/A275 determinada a 0,5-1,0 mg/ml en agua desionizada. Es una medida del contenido de hemina, no de la actividad enzimática. Incluso preparaciones con RZ elevada pueden tener una actividad enzimática baja.

Other Notes

Véase más información sobre la peroxidasa en www.sigma-aldrich.com/enzymeexplorer.

inhibitor

Referencia del producto
Descripción
Precios

S2002

Acida sódica, ReagentPlus®, ≥99.5%

substrate

Referencia del producto
Descripción
Precios

D9143

o-Dianisidine, peroxidase substrate

A0156

N-(4-Aminobutyl)-N-ethylisoluminol, ≥90%

A4685

Luminol sodium salt

A1888

Ácido 2,2′-acino-bis(3-etilbenzotiazolina-6-sulfónico) diammonium salt, ≥98% (HPLC)

G5502

Guaiacol, oxidation indicator

A1661

N-(6-Aminohexyl)-N-ethylisoluminol

A3537

5-Aminosalicylic acid, ≥99%

A4382

4-Aminoantipyrine, reagent grade

A8264

4-Aminophthalhydrazide

A5754

3-Amino-9-ethylcarbazole, ≥95% (HPLC), powder

A8511

Luminol, ≥97% (HPLC)

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

ppe

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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D W Potter et al.
Molecular pharmacology, 29(2), 155-162 (1986-02-01)
Horseradish peroxidase catalyzed the polymerization of acetaminophen. Addition of reduced glutathione (GSH) to reaction mixtures resulted in decreased polymerization and formation of minor amounts of GSH-acetaminophen conjugates. The conjugates were identified as 3-(glutathion-S-yl)acetaminophen and 3-(glutathion-S-yl)diacetaminophen. Horseradish peroxidase also catalyzed polymerization
Yanqin Wen et al.
Analytical chemistry, 84(18), 7664-7669 (2012-08-30)
We present a highly sensitive colorimetric method for microRNA (miRNA) detection. This method is based on a rolling-circle amplification (RCA) DNA machine, which integrates RCA, nicking enzyme signal amplification and DNAzyme signal amplification. The DNA machine is triggered by the
Keehoon Won et al.
Biomacromolecules, 5(1), 1-4 (2004-01-13)
Horseradish peroxidase-catalyzed polymerization of cardanol in aqueous organic solvent was investigated in the presence of a redox mediator. Cardanol is a phenol derivative from a renewable resource mainly having a C15 unsaturated hydrocarbon chain with mostly 1-3 double bonds at
Sun-Joo Moon et al.
Phytochemistry, 82, 15-21 (2012-08-14)
In this study, dehydrogenative polymers (DHP) were synthesized in vitro through dehydrogenative polymerization using different ratios of coniferyl alcohol (CA) and sinapyl alcohol (SA) (10:0, 8:2, 6:4, 2:8, 0:10), in order to investigate the monolignol coupling mechanism in the presence
Jane M Natividad et al.
PloS one, 4(7), e6472-e6472 (2009-08-04)
Excessive uptake of commensal bacterial antigens through a permeable intestinal barrier may influence host responses to specific antigen in a genetically predisposed host. The aim of this study was to investigate whether intestinal barrier dysfunction induced by indomethacin treatment affects
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Protocolos

Spectrophotometric Determination of Reinheitszahl (RZ) for Peroxidase (EC 1.11.1.7)

Reinheitszahl (RZ) is the ratio of absorbance due to hemin (A403, Soret region) to absorbance due to protein (A275).

Spectrophotometric Determination of Reinheitszahl (RZ) for Peroxidase (EC 1.11.1.7)

Reinheitszahl (RZ) is the ratio of absorbance due to hemin (A403, Soret region) to absorbance due to protein (A275).

Spectrophotometric Determination of Reinheitszahl (RZ) for Peroxidase (EC 1.11.1.7)

Reinheitszahl (RZ) is the ratio of absorbance due to hemin (A403, Soret region) to absorbance due to protein (A275).

Spectrophotometric Determination of Reinheitszahl (RZ) for Peroxidase (EC 1.11.1.7)

Reinheitszahl (RZ) is the ratio of absorbance due to hemin (A403, Soret region) to absorbance due to protein (A275).

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