N1905

Neurotrophin-3 human

recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture, >97% (SDS-PAGE)

• Sinónimos: HDNF, NTF3, NT-3
• Número MDL: MFCD00212404
• Código UNSPSC: 12352202
• NACRES: NA.32

Propiedades

• origen biológico: human
• Nivel de calidad: 200
• recombinante: expressed in baculovirus infected Sf21 cells
• Ensayo: >97% (SDS-PAGE)
• Formulario: lyophilized powder
• potencia: 1-40 ng/mL EC₅₀
• envase: pkg of 5 μg
• técnicas: cell culture | mammalian: suitable
• impurezas: endotoxin, tested
• Nº de acceso UniProt: P20783
• temp. de almacenamiento: −20°C
• Información sobre el gen: human ... NTF3(4908)

Descripción

Descripción general

NTF3 (neurotrophin 3) is a neurotrophic factor belonging to the NGF (nerve growth factor) family. This protein is made up of 257 amino acids, with a prepro region of 138 amino acids and the mature sequence of 119 residues.

Aplicación

Neurotrophin-3 human has been used in the magnetic sorting of oligodendrocyte progenitor cells.

Acciones bioquímicas o fisiológicas

Neurotrophin-3 (13.6 kDa or 27.2 kDa dimer) is a member of the neurotrophin family. It has identical amino acid sequence in human, mouse, and pig with full cross-reactivities. NT-3 is important in development and maintenance of neuronal populations and promotes differentiation of neural crest derived sensory and sympathetic neurons. It is critical for proprioceptive 1a afferent neurons, which relay information from peripheral muscle spindles to motoneurons, sending projections to spinocerebellar neurons. NT-3 is also critical in the superior cervical and nodose ganglia.

Forma física

Lyophilized from a 0.2 m filtered solution in phosphate buffered saline (PBS) containing 50 μg of bovine serum albumin (BSA) per 1 μg of cytokine.

Nota de análisis

The biological activity is measured in a cell proliferation assay using a TrkB-transfected cell line BaF-TrkB-BD.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)