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Merck
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Documentos

M5310

Sigma-Aldrich

Aceite de inmersión

light oil, suitable for mouse embryo cell culture, BioXtra

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About This Item

Número de CAS:
8042-47-5
EC Number:
232-455-8
MDL number:
MFCD00131611
UNSPSC Code:
12352207
NACRES:
NA.75

sterility

sterile-filtered

product line

BioXtra

form

light oil

storage condition

protect from light

technique(s)

cell culture | embryo: suitable

refractive index

n20/D 1.467 (lit.)

density

0.82-0.88 g/mL at 25 °C

format

neat

InChI key

AEOVEGJBKQQFOP-DDVLFWKVSA-L

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General description

Mineral oil or liquid petroleum is used for conservation of various types of cutltures. The advantages of using mineral oil are that the growth rate is decreased and the evaporation of water from the medium is almost completely eliminated. Thus, sub-culturing is reduced leading to increase in the viability of the culture. The first time mineral oil was used was in 1914, and it was used to overlay bacterial culture.

Application

Mineral oil has been commonly used and recommended for overlaying mouse embryo cultures to prevent drying during the procedure.
Mineral oil is used for the following applications:
  • Used for cell viability studies in preparing the culture media or media supplements
  • Used tp protect array from evaporation by adding mineral oil around the array
  • Used to prepare the needle for microinjection (microinjection needle pulling, sharpening, and loading)
  • Used in PCR setup

Features and Benefits

Ready-to-use sterile filtered product conveniently packaged in PET bottles.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

No data available

flash_point_c

No data available

ppe

Eyeshields, Gloves

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Mineral Oil Overlay for Conservation of Plant Tissue Cultures
Caplin SM
American Journal of Botany, 46(5), 324-329 (1959)
Slobodan Beronja et al.
Methods in molecular biology (Clifton, N.J.), 961, 351-361 (2013-01-18)
We have recently developed a method for RNAi-mediated gene function analysis in skin (Beronja et al., Nat Med 16:821-827, 2010). It employs ultrasound-guided in utero microinjections of lentivirus into the amniotic cavity of embryonic day 9 mice, which result in
Bei Zhao et al.
Scientific reports, 7(1), 11319-11319 (2017-09-14)
A microfluidic chip with a controllable and integrated piezoelectric pump was proposed and demonstrated, where the pump was designed as a micro-actuator based on polyvinylidene fluoride (PVDF) organic piezoelectric film. In this case, the pump should integrate with the microfluidics
Hanyoup Kim et al.
Lab on a chip, 9(9), 1230-1235 (2009-04-17)
We report high-speed real-time PCR performed on an unmodified disposable polystyrene Petri dish. The reaction cycle relies solely on an infrared laser for heating; no conventional heater is required. Nanoliter droplets of PCR mixture as water-in-oil emulsions printed in an
Xiyan Wang et al.
Journal of clinical laboratory analysis, 34(11), e23498-e23498 (2021-01-20)
Efficiency of preantral follicle culture in vitro is low and is dependent on species, development stage, and follicle-stimulating hormone (FSH) concentration. Here, we optimized the preantral follicle in vitro culture system in mice. The primary follicles (PM follicles, 80-100 μm
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