M0275

Medio esencial mínimo Eagle

With Earle′s salts, without ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture, 10x

• Sinónimos: MEM
• UNSPSC Code: 12352207
• NACRES: NA.71

Propiedades

• product name: Medio esencial mínimo Eagle, 10 ×, With Earle′s salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
• Quality Level: 400
• sterility: sterile-filtered
• form: liquid
• concentration: 10 ×
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• components: phenol red: yes; HEPES: no; Earle’s salts (5% CO₂): yes; L-glutamine: no; sodium pyruvate: no; NaHCO₃: no
• shipped in: ambient
• storage temp.: 2-8°C

Descripción

Application

Generally used to grow attached cell lines, such as fibroblasts, in the presence of FBS, calf or horse sera. The L-glutamine and sodium bicarbonate are added to the medium after dilution to 1x and pH adjustment.

Minimum Essential Medium Eagle has been used for the formulation of collagen methacrylamide (hydrogel). It has also been used to maintain articular cartilage of bovine knee joints, which was later used for MRI (magnetic resonance imaging) study and histological analysis.

Reconstitution

Supplement with 0.292 g/L L-glutamine, 2.2 g/L sodium bicarbonate at 1×.

Información de seguridad

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable