G9637

Glycerol 3-phosphate Oxidase from Pediococcus sp.

lyophilized powder, 40-80 units/mg solid, pH 8.1

• Sinónimos: sn-Glycerol 3-phosphate:oxygen 2-oxidoreductase, L-Glycerol 3-phosphate Oxidase
• Número de CAS: 9046-28-0
• Comisión internacional de enzimas: 1.1.3.21 (BRENDA, IUBMB)
• EC Number: 232-932-0
• MDL number: MFCD00131209
• UNSPSC Code: 12352204
• NACRES: NA.54

Propiedades

• biological source: bacterial (Pediococcus spp.)
• form: lyophilized powder
• specific activity: 40-80 units/mg solid, pH 8.1
• mol wt: ~76 kDa (gel filtration)
• storage temp.: 2-8°C

Descripción

General description

Glycerol 3-phosphate Oxidase is a FAD-dependent α-glycerophosphate oxidase and catalyzes the formation of dihydroxyacetonephosphate. Glycerol 3-phosphate oxidase comprises of a flavin adenine dinucleotide (FAD) and a substrate binding domain and corresponds to molecular weight of 42 kDa. It exists as a monomer and the active site comprises of isoalloxazine ring and histidine 53, arginine 316 and lysine 345 residues crucial for ligand binding.

Application

Glycerol 3-phosphate Oxidase from Pediococcus sp. has been used in the kinetic assay to study gluconeogenesis in liver and in immobilization studies on pencil graphite (PG) electrode.

This enzyme is useful for enzymatic determination of triglyceride when coupled with lipoprotein lipase (LPL-311, LPL-314) and glycerokinase (GYK-301, GYK-311) in clinical analysis.

Physical properties

Isoelectric point : 4.1 -/+0.1
Michaelis constants : 3.2x10 ^-3M (L-α-Glycerophosphate), 6.8 x 10 ^-3M (D, L-form)
Inhibitors : Ionic detergents (SDS, LBS, etc.), Hg⁺⁺, Ag⁺
Optimum pH : 8.0 - 8.5Optimum temperature : 35 - 40o^C
pH Stability : pH 6.5 - 8.5 (25o^C, 20hr) Thermal stability : below 40oC (pH 7.0, 15min)
Substrate specificity : The enzyme has the highest specificity for L-form of a-γlycerophosphate

Unit Definition

One unit will oxidize 1.0 μmole of L-glycerol 3-phosphate to dihydroxyacetone phosphate with the formation of H₂O₂ per min at 37°C, at the appropriate pH.

Physical form

Lyophilized powder containing stabilizers

Información de seguridad

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)